Anti-TFB1M Rabbit Polyclonal Antibody for WB, ELISA - Q8WVM0

Core Product Specifications and Parameters

Biological Background: TFB1M Function and Localization

• TFB1M, dimethyladenosine transferase 1, mitochondrial, is an S-adenosylmethionine-dependent methyltransferase that dimethylates adjacent adenosines A1583 and A1584 in the loop of helix 45 of 12S rRNA, a modification essential for the assembly or stability of the small mitochondrial ribosomal subunit. Related references: PMID:12496758, PMID:25305075, PMID:31251801
• It also plays a role in basal transcription of mitochondrial DNA by interacting with POLRMT and TFAM, and this transcriptional stimulation is independent of its methyltransferase activity. Related references: PMID:11809803, PMID:12068295, PMID:12897151
• The protein is located in the mitochondrion.
• TFB1M is ubiquitously expressed in human tissues.
• It belongs to the methyltransferase family and contains domains for S-adenosyl-L-methionine binding, RNA binding, and DNA binding, functioning in rRNA processing, transcription regulation, and mitochondrial gene expression.
• Alternative names include CGI75, mtTFB, CGI-75, mtTFB1, and h-mtTFB1.
• The precursor protein includes a transit peptide for mitochondrial targeting; the mature enzyme is approximately 40 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 28–346 of human TFB1M, which excludes the N‑terminal mitochondrial targeting sequence; thus the antibody may detect both precursor and mature forms. In Western blot, a band around 40 kDa is expected, consistent with the calculated molecular weight of the processed protein.
• For Western blot, mitochondrial‑enriched fractions from human, mouse, or rat cell or tissue lysates may provide optimal detection. Validate the antibody in your specific sample type and lysis conditions.
• The antibody is qualified for ELISA; consider using the recombinant immunogen as a positive control when designing assays.
• Due to TFB1M’s dual roles in mitochondrial rRNA modification and transcription, functional studies (e.g., methyltransferase activity assays or transcription reporter systems) can complement detection by western blot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one‑stop access to IVD raw materials, including this Anti‑TFB1M Rabbit Polyclonal Antibody for mitochondrial biology investigations. We facilitate sourcing of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to accelerate your assay development—from concept to clinic.