Anti-TFAP2C Polyclonal Antibody for WB, ELISA - Q92754

Core Product Specifications and Parameters

Biological Background: TFAP2C Function and Localization

• TFAP2C (Transcription factor AP-2 gamma), also known as Activating enhancer-binding protein 2 gamma or ERF-1, is a sequence-specific DNA-binding transcription factor.
• It binds to the consensus sequence 5'-GCCNNNGGC-3' and activates genes involved in a broad range of biological functions, including embryonic development and cancer progression. Related references: PMID:11694877 PMID:24413532
• TFAP2C plays a critical role in early embryonic development by regulating inner cell mass (ICM) and trophectoderm differentiation, as well as controlling cell-polarity genes during morula development.
• In trophoblast commitment, it binds to late trophectoderm genes together with CDX2, and later to extra-embryonic ectoderm genes with SOX2.
• TFAP2C is involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Related references: PMID:24413532
• Subcellular localization: Predominantly located in the nucleus.
• Key features include DNA-binding, transcription activation, and post-translational modifications such as phosphorylation, ubiquitination, and isopeptide bond formation.
• It exists in alternatively spliced isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to residues 350-450, which may confer specificity for this region; verify reactivity in the target species.
• For Western blot, the predicted molecular weight is ~49 kDa; consider using appropriate controls and optimizing lysis conditions to preserve epitope accessibility.
• For ELISA, titrate the antibody and optimize coating conditions to achieve optimal signal-to-noise ratio.
• Validate cross-reactivity in human, mouse, and rat samples as per experimental requirements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes one-stop access to IVD raw materials, technical services, and consulting—supporting every phase from concept to clinic. This anti-TFAP2C polyclonal antibody is well-suited for research in transcription regulation, developmental biology, and oncology. Beyond this product, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your IVD development.