Anti-TFAP2B Polyclonal Antibody for WB, ELISA - Q92481

Core Product Specifications and Parameters

Biological Background: TFAP2B Function and Localization

• Transcription factor AP-2-beta is a sequence-specific DNA-binding protein that recognizes the consensus motif 5'-GCCNNNGGC-3' to regulate target gene transcription.
• It functions as both a transcriptional activator and repressor, activating genes critical for embryonic development (eye, face, body wall, limb, neural tube) and suppressing genes such as MCAM/MUC18, C/EBP alpha, and MYC.
• AP-2-beta is essential for normal craniofacial and limb morphogenesis, and for terminal differentiation and function of renal tubular epithelia.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications include phosphorylation and ubiquitin conjugation, which may modulate its activity and stability.
• Alternative splicing produces multiple transcript variants, potentially diversifying its functional repertoire.
• Belongs to the AP-2 family of transcription factors, characterized by a conserved DNA-binding domain.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (amino acids 1–100) of human TFAP2B; consider using peptide competition assays to confirm specificity.
• For Western blot, a band at approximately 50 kDa is expected; include appropriate loading controls and verify with knockout or knockdown models if available.
• In ELISA applications, optimize coating conditions using synthetic peptide or recombinant TFAP2B protein to ensure consistent performance.
• Evaluate the antibody in relevant nuclear extracts or whole-cell lysates from human cell lines expressing TFAP2B.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with a comprehensive range of IVD raw materials for transcriptional regulation and developmental biology studies. Our portfolio features validated polyclonal antibodies like anti-TFAP2B, along with optimized antibody pairs, recombinant proteins, and ancillary reagents. For bulk orders, rare target sourcing, or tailored technical consultation, contact our team to accelerate your assay development from concept to clinic.