Anti-TFAM Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q00059

Core Product Specifications and Parameters

Biological Background: TFAM Function and Localization

• Transcription factor A, mitochondrial (TFAM, also known as MtTF1, TCF-6, TCF6L2) is a key regulator of mitochondrial DNA (mtDNA) transcription and maintenance.
• Binds to the mitochondrial light strand promoter to regulate transcription initiation; essential component of the mitochondrial transcription initiation complex with TFB2M and POLRMT. Related references: PMID:29149603
• Promotes transcription from both the heavy strand promoter 1 (HSP1) and the light strand promoter by binding immediately upstream of start sites, and can unwind DNA. Related references: PMID:22037172
• Bends promoter DNA into a U-turn shape via its HMG-box domains, facilitating RNA polymerase recruitment. Related references: PMID:1737790
• Required for mtDNA maintenance and copy number regulation; plays a role in organizing and compacting mitochondrial nucleoids. Related references: PMID:19304746, PMID:22841477, PMID:22037171
• Localizes to the mitochondrial matrix and mitochondrial nucleoid.
• Post-translationally modified by phosphorylation; classified as a phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100-200 of human TFAM; for applications requiring native conformation (e.g., IF/ICC), verify that the epitope is accessible.
• For Western blotting, the predicted molecular weight is approximately 29 kDa, but validate in your sample system.
• When using for IHC-P, optimize antigen retrieval and antibody dilution; cross-reactivity with mouse and rat tissues should be confirmed.
• As a polyclonal antibody, lot-to-lot consistency should be validated for critical assays; consider using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For mitochondrial research targets like TFAM, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and support for rare target raw-material sourcing. Partner with CamelBio to streamline your assay development and procurement.