Anti-TFAM Rabbit Monoclonal Antibody for WB - Q00059

Core Product Specifications and Parameters

Biological Background: TFAM Function and Localization

• TFAM (Transcription Factor A, Mitochondrial; also known as MtTF1, TCF-6) is a key mitochondrial protein that regulates mitochondrial DNA (mtDNA) transcription and maintenance.
• It binds to the mitochondrial light strand promoter and is a component of the mitochondrial transcription initiation complex, together with TFB2M and POLRMT, to enable basal transcription; it also promotes transcription initiation from the HSP1 and light strand promoters by binding immediately upstream of transcriptional start sites. Related references: PMID:29149603 PMID:22037172
• TFAM recruits POLRMT to specific promoters, facilitating accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Related references: PMID:20410300
• The protein unwinds DNA and bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes, aiding transcription initiation. Related references: PMID:22037172 PMID:1737790
• Beyond transcription, TFAM is essential for maintaining normal mtDNA copy number and may play a role in organizing and compacting mitochondrial DNA. Related references: PMID:19304746 PMID:22841477 PMID:22037171
• Subcellularly localized to the mitochondrion matrix and mitochondrial nucleoid, consistent with its role in mtDNA metabolism.
• Post-translational modifications include phosphorylation, and alternative splicing produces multiple isoforms of TFAM.
• Clinically, mutations in TFAM are associated with primary mitochondrial disease (e.g., MTDPS15).

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-100) of human TFAM; therefore, the antibody may detect full-length protein and isoforms containing this region. Validate reactivity in your sample system.
• For chromatin immunoprecipitation (ChIP), consider that TFAM localizes to mitochondrial nucleoids; ChIP protocols may require adaptation for mitochondrial DNA-protein complexes.
• Optimal working dilutions for each application should be determined empirically; typical starting concentrations can be guided by the supplier's general monoclonal antibody recommendations.
• Although cross-reactivity is listed as human only, you may test on closely related species if sequence homology is high in the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials for mitochondrial research, including validated TFAM antibodies. As a trusted supply partner, we offer optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support from concept to clinic. Contact us for rare target sourcing and assay-specific solutions.