Anti-TET2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q6N021

Core Product Specifications and Parameters

Biological Background: TET2 Function and Localization

• TET2 is a methylcytosine dioxygenase that catalyzes the iterative oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), playing a key role in active DNA demethylation.
• The enzyme preferentially acts on 5-hydroxymethylcytosine in CpG motifs, and its activity is critical for epigenetic regulation of transcription in mammalian genomes.
• TET2 also has a non-catalytic function, recruiting O-GlcNAc transferase (OGT) to CpG-rich transcription start sites, thereby promoting histone H2B GlcNAcylation and influencing gene expression.
• The protein localizes to the nucleus and associates with chromosomes, consistent with its role in chromatin modification and DNA demethylation.
• TET2 is broadly expressed but shows highest levels in hematopoietic cells, particularly granulocytes; its expression is notably reduced in granulocytes from patients with myelodysplastic syndromes (MDS).
• The protein features multiple domains and modifications, including a dioxygenase domain, iron- and zinc-binding sites, and regulatory post-translational modifications such as acetylation, phosphorylation, and O-GlcNAcylation.
• TET2 is implicated as a tumor suppressor, with genetic alterations frequently observed in myeloid malignancies and other cancers, highlighting its importance in hematopoietic differentiation and genomic stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 1833–2002) of the long isoform of human TET2; this antibody is expected to recognize full-length TET2 but may not detect N-terminal truncations or isoforms lacking this region.
• For Western blotting, given the large predicted molecular weight (224 kDa), consider using low-percentage polyacrylamide gels and extended transfer times to ensure efficient membrane transfer.
• In cell or tissue staining applications (IHC-P, IF/ICC), be aware that TET2 is primarily nuclear; appropriate permeabilization and nuclear counterstaining may aid in signal localization.
• Validate the antibody’s reactivity in the relevant cell type or tissue, especially given the tissue-specific expression and disease-related downregulation in hematopoietic samples.
• Optimize antibody dilutions for each application using a known positive control (e.g., cells with confirmed TET2 expression), as quantitative performance data are not provided.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering a seamless bridge from concept to clinic. This TET2 rabbit polyclonal antibody is particularly suited for epigenetic and cancer research applications, and we can support your projects with validated antibody pairs, bulk ancillary reagents, and tailored sourcing for rare targets. Whether you need optimized monoclonal antibodies, calibration-grade materials, or custom procurement, CamelBio streamlines your supply chain to accelerate diagnostic development.