Anti-TET1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q3URK3

Core Product Specifications and Parameters

Biological Background: TET1 Function and Localization

• TET1 (Methylcytosine dioxygenase TET1) catalyzes the sequential oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), serving as a key enzyme in active DNA demethylation.
  Related references: PMID:20639862, PMID:21496894, PMID:21778364

• Beyond demethylation, TET1 recruits histone-modifying complexes to alter chromatin accessibility, thereby functioning as both a transcriptional activator and repressor in processes such as stem cell maintenance, immune cell development, and neural activity.
  Related references: PMID:28504700, PMID:32286661

• TET1 localizes predominantly to the nucleus and associates with chromatin, consistent with its central roles in DNA modification and epigenetic regulation.

• In early development, TET1 is critical for embryonic stem cell self-renewal and inner cell mass specification, balancing pluripotency and lineage commitment.
  Related references: PMID:20639862, PMID:28504700

• The mouse Tet1 gene produces at least two major isoforms: isoform 1 is highly expressed in early embryos and ES cells, while isoform 2 is predominant in differentiated tissues and shows distinct chromatin-binding properties and functional outcomes, including differential regulation of synaptic transmission and memory.
  Related references: PMID:27916660, PMID:33262245, PMID:36056023

• TET1 contributes to epigenetic reprogramming of pericentromeric heterochromatin in germ cells and is required for normal meiotic progression in oocytes.
  Related references: PMID:34166371, PMID:23151479

• TET1 expression is detected in a variety of tissues, with higher levels in oocytes, early embryos, ES cells, and brain; isoform-specific expression patterns exist, with isoform 1 enriched in glial cells and isoform 2 in neurons.
  Related references: PMID:20639862, PMID:24357321, PMID:28531272, PMID:33262245

• TET1 is subject to multiple post-translational modifications (phosphorylation, glycosylation, ubiquitination) and contains essential zinc-finger domains for DNA binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fusion protein covering amino acids 1749–1902 of mouse TET1 isoform 1 (NP_001240786.1), a region that may be conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, a band of approximately 219 kDa is expected; however, due to the presence of isoforms and potential post-translational modifications, validate the detected band using appropriate controls and molecular weight markers.
• IHC-P application may require optimization of antigen retrieval and detection conditions, as TET1 expression can be low or restricted to specific cell types in many tissues.
• As a polyclonal antibody, lot-to-lot variability can occur; consider including positive and negative controls and performing titration experiments for each new lot.
• ELISA usage has been reported; ensure the coating antigen and detection system are compatible, and validate with known positive and negative samples.
• For applications involving isoform distinction, be aware that the epitope region may be present in multiple isoforms; verify specificity using isoform-specific knockdown or overexpression systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing platform for IVD raw materials, covering every stage from concept to clinical application. With this TET1 polyclonal antibody, researchers studying DNA demethylation and chromatin biology can accelerate their assay development. CamelBio also offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing solutions for rare targets—ensuring reliable supply for diagnostic manufacturers and research laboratories.