Anti-TET1 Monoclonal Antibody for WB and ELISA - Q8NFU7

Core Product Specifications and Parameters

Biological Background: TET1 Function and Localization

• TET1 (also known as CXXC6, LCX, or Ten-eleven translocation 1 gene protein) is a methylcytosine dioxygenase and member of the TET protein family that plays a pivotal role in active DNA demethylation through the sequential oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). Related references: PMID:19372391 PMID:21496894 PMID:21778364
• Beyond its catalytic demethylation activity, TET1 functions as a chromatin regulator by recruiting histone-modifying protein complexes, thereby altering histone marks and chromatin accessibility, which can lead to both activation and repression of gene expression. Related references: PMID:33833093
• TET1 is involved in diverse biological processes, including embryonic stem cell maintenance, T- and B-cell development, inflammation regulation, genomic imprinting, neural activity, and DNA repair. Related references: PMID:31278917
• Tissue expression is observed in fetal heart, lung, and brain, as well as adult skeletal muscle, thymus, and ovary, but not in adult heart, lung, or brain; protein levels are up-regulated in glioblastoma cells. It is also expressed in embryonic stem cells. Related references: PMID:25284789
• Subcellular localization is primarily nuclear, with association to chromosomes, reflecting its direct role in DNA and chromatin modification.
• Alternative splicing produces multiple isoforms with distinct chromatin-binding properties and roles; for instance, isoform 1 and isoform 2 differentially regulate gene expression and synaptic transmission in hippocampal neurons.
• The protein features a CXXC-type zinc-finger domain for DNA binding, a catalytic domain requiring iron as a cofactor, and undergoes post-translational modifications such as phosphorylation and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 1800–1900 of human TET1, suggesting the antibody likely recognizes the C-terminal portion of the protein; consider verifying specificity with appropriate controls in your sample system.
• With a predicted molecular weight of approximately 235 kDa, use a low-percentage (e.g., 6–8%) SDS-PAGE gel and extended transfer times for Western blotting to ensure efficient transfer of this high-molecular-weight protein.
• The antibody has been shown to react with human, mouse, and rat samples; validate cross-reactivity in your specific experimental context, especially when using mouse or rat tissues.
• For ELISA applications, ensure proper antibody titration and blocking conditions, as the recombinant monoclonal format may exhibit high affinity and require optimization to reduce background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a comprehensive one-stop platform for IVD raw materials, offering validated monoclonal antibodies like this TET1 Rabbit mAb, optimized antibody pairs, and bulk ancillary reagents. Our supply chain expertise supports customers working on epigenetic targets and rare analytes, ensuring timely access to high-quality raw materials for immunoassay development and biomarker research. Whether you need routine or custom-sourced raw materials, CamelBio bridges the gap from concept to clinic with reliable, scalable solutions.