Anti-TERT Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O14746

Core Product Specifications and Parameters

Biological Background: TERT Function and Localization

• Telomerase reverse transcriptase (TERT) is the catalytic component of telomerase, a ribonucleoprotein enzyme that maintains telomere ends by adding TTAGGG repeats. It is active in progenitor and cancer cells, but inactive or very low in normal somatic cells.
• Functions as an RNA-dependent DNA polymerase, catalyzing the extension of 3'-chromosomal termini using an internal RNA template. The catalytic cycle involves primer binding, extension, and product release or translocation, and it is more active on substrates with 2 or 3 telomeric repeats.
• TERT activity is regulated by telomerase complex-associated proteins, chaperones, and polypeptide modifiers. It also modulates Wnt signaling and plays important roles in aging and antiapoptosis.
• Alternative names include TP2, TRT, CMM9, EST2, TCS1, hTRT, DKCA2, DKCB4, hEST2, and PFBMFT1. The gene TERT has synonyms EST2, TCS1, and TRT.
• Subcellular localization is primarily nuclear, including the nucleolus, nucleoplasm, and telomeres, but it also localizes to PML bodies and the cytoplasm.
• Post-translational modifications include phosphorylation and ubiquitin conjugation. Keywords associate TERT with telomere maintenance, reverse transcription, and disease variants such as dyskeratosis congenita.
• Tissue specificity: highly expressed in thymocyte subpopulations, intermediate in tonsil T-lymphocytes, and low to undetectable in peripheral blood T-lymphocytes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 1050-1132 of human TERT, targeting a C‑terminal region. Consider this epitope location when designing complementary detection reagents.
• For WB, TERT has a predicted molecular weight of ~127 kDa. Use reducing conditions and heating to dissociate complexes. Note that phosphorylated or modified forms may migrate differently; include appropriate cancer cell line lysates as positive controls.
• In IHC‑P, anticipate nuclear and nucleolar staining patterns in telomerase‑positive tissues. Optimize antigen retrieval methods and validate with known positive and negative tissue samples.
• For ELISA, this polyclonal antibody may be used as capture or detection antibody depending on the assay format. Pairing with a validated monoclonal antibody can improve specificity in sandwich ELISA setups.
• As a polyclonal antibody, lot‑to‑lot variability should be assessed by the end user. Validate performance in the specific sample system and application before routine use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one‑stop access to IVD raw materials, including this anti‑TERT polyclonal antibody for telomerase‑associated disease research. Beyond antibodies, we supply validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and rare‑target sourcing to support your full development pipeline. Let us bridge your concept to clinic with reliable, scalable raw‑material solutions.