Anti-TERF2IP Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NYB0

Core Product Specifications and Parameters

Biological Background: TERF2IP Function and Localization

• TERF2IP (also known as RAP1, DRIP5) is a component of the shelterin complex, which protects telomere ends and regulates telomere length.
• It negatively regulates telomere recombination by repressing homology-directed repair (HDR), thereby maintaining telomere length homeostasis; it is recruited to telomeric TTAGGG repeats via interaction with TERF2.
• Beyond telomeres, TERF2IP acts as a transcription regulator at extratelomeric TTAGGG sites, modulating gene expression through association with TERF2 or other factors.
• In the cytoplasm, TERF2IP associates with the IKK complex and promotes NF-κB signaling by enhancing IKK-mediated phosphorylation of RELA/p65, leading to activation of NF-κB target genes.
• Subcellular localization includes the nucleus (telomeres and extratelomeric sites), cytoplasm, and chromosome territories.
• The protein is ubiquitously expressed at high levels across human tissues.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation (isopeptide bond).

Experimental Guidance and Technical Tips

• The immunogen (aa 1–280) covers the N-terminal region of TERF2IP; consider verifying antibody specificity with recombinant protein or lysates from TERF2IP-knockdown/knockout cells.
• For Western blot, TERF2IP runs at approximately 44 kDa; validate using human or mouse cell lysates and appropriate loading controls.
• In immunofluorescence/immunocytochemistry, expect both nuclear and cytoplasmic signals; include no-primary and blocking controls to assess specificity.
• Optimize dilution and incubation conditions in your specific experimental system; preliminary titration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement platform for IVD manufacturing and research, delivering validated antibodies, bulk reagents, and technical support. For telomere biology and NF-κB signaling studies, we source this TERF2IP polyclonal antibody alongside a wide range of shelterin components and transcription factor reagents. Our integrated supply chain ensures reliable access to raw materials from concept to clinic.