Anti-TERF2 Polyclonal Antibody for WB - Q15554

Core Product Specifications and Parameters

Biological Background: TERF2 Function and Localization

• Telomeric repeat-binding factor 2 (TERF2), also known as TRF2, is a DNA-binding protein that specifically binds the telomeric double-stranded 5'-TTAGGG-3' repeat to maintain telomere integrity and prevent chromosome end fusion. Related references: PMID:15608617 PMID:16166375 PMID:20655466
• TERF2 is a core component of the shelterin complex, which associates with telomeric repeats to regulate telomere length and protect chromosome termini from being recognized as DNA damage. Related references: PMID:16166375
• TERF2 recruits the shelterin complex members TERF2IP/RAP1 and the nuclease DCLRE1B/Apollo to telomeres, enabling proper telomere protection and processing. Related references: PMID:16166375 PMID:20655466
• Together with DCLRE1B/Apollo, TERF2 is essential for forming telomeric loops (T-loops) by generating 3' overhangs, a structure critical for chromosome end protection. Related references: PMID:20655466
• TERF2 controls DNA topoisomerase activity (TOP1, TOP2A, TOP2B) during telomere replication to prevent aberrant topology. Related references: PMID:20655466
• TERF2 preferentially binds positive supercoiled DNA, potentially contributing to its capacity to facilitate T-loop and alternative structures. Related references: PMID:15608617 PMID:20655466
• TERF2 localizes to the nucleus and specifically accumulates at telomeres, consistent with its function in chromosome end maintenance.
• TERF2 is ubiquitously expressed, with higher levels in spleen, thymus, prostate, uterus, testis, small intestine, colon, and peripheral blood leukocytes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 200-300 of human TERF2 (NP_005643.2); this internal region is well-conserved in mouse and rat, supporting the reported cross-reactivity. Nevertheless, validation in the specific experimental system is advised.
• For Western blotting, a dilution range of 1:500 to 1:2000 is a reasonable starting point; conditions should be optimized for each sample type and detection system. The predicted molecular weight is approximately 60 kDa.
• In ELISA, the antibody can be utilized as a capture or detection reagent; titering is recommended to achieve optimal signal and specificity.
• As a polyclonal antibody, lot-to-lot variability may occur; consider including positive and negative controls and, if possible, lot-specific optimization for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide a comprehensive suite of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents, streamlining the transition from concept to clinic. This anti-TERF2 polyclonal antibody supports telomere and shelterin complex research, complementing CamelBio's portfolio of high-quality immune reagents. Contact our team to discuss your specific antibody sourcing, customization, or technical support needs.