Anti-TERF1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P54274

Core Product Specifications and Parameters

Biological Background: TERF1 Function and Localization

• TERF1, also known as TRF1, PIN2, TRBF1, specifically binds the double-stranded 5'-TTAGGG-3' telomeric repeat and negatively regulates telomere length, preventing excessive elongation. Related references: PMID:31595153
• It is a core component of the shelterin (telosome) complex, which protects chromosome ends from inappropriate DNA damage responses and maintains genomic integrity.
• TERF1 participates in mitotic spindle regulation, linking telomere maintenance to cell division processes.
• The protein localizes to the nucleus, particularly at chromosome telomeres, and can also be found in the cytoplasm, associated with the cytoskeleton and spindle apparatus.
• TERF1 undergoes various post-translational modifications, including acetylation, phosphorylation, and ADP-ribosylation, which may modulate its function and protein interactions.
• It is highly and ubiquitously expressed, with the Pin2 isoform being predominant.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 52-275 of human TERF1, a central region that may allow detection of multiple isoforms or modification states; validate specificity in your experimental system.
• For Western blot, expect a band near 50 kDa; use telomere-positive cell lines (e.g., HeLa) as positive controls.
• In IHC-P, TERF1 typically yields nuclear staining; consider co-staining with a telomere marker or DNA damage marker to confirm localization.
• This antibody is reported to cross-react with rat; when using rat tissues, optimize antigen retrieval and antibody dilution to achieve reliable results.
• For ELISA, employ a standard curve and include both positive and negative controls to ensure assay performance within the linear range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with efficient access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. In telomere biology and chromosome stability research, we help you source high-quality TERF1 antibodies and related tools to streamline your assay development. Contact our team to leverage our comprehensive supply chain — from concept to clinic.