Anti-Tenascin-X (TNXB) Rabbit Polyclonal Antibody for WB, ELISA - P22105

Core Product Specifications and Parameters

Biological Background: Tenascin-X Function and Localization

• Tenascin-X, also known as Hexabrachion-like protein, is an extracellular matrix glycoprotein encoded by the TNXB gene.
• It mediates interactions between cells and the extracellular matrix and acts as a substrate-adhesion molecule that inhibits cell migration.
• Accelerates collagen fibril formation, contributing to the structural integrity of connective tissues.
• May play a role in supporting the growth of epithelial tumors.
• Contains characteristic EGF-like domains, fibronectin type-III repeats, and a C-terminal fibrinogen-like domain; undergoes alternative splicing to produce tissue-specific isoforms.
• Isoform XB-short is specifically expressed in the adrenal gland, while full-length protein is highly expressed in fetal adrenal, testis, and striated/cardiac muscle.
• Secreted into the extracellular space and localized within the extracellular matrix.
• Genetic variants in TNXB are associated with Ehlers-Danlos syndrome, highlighting its importance in connective tissue stability.

Experimental Guidance and Technical Tips

• The immunogen lies within amino acids 444–673 of human TNXB (NP_115859.2); verify domain coverage in your experimental context.
• For Western blot, consider the large predicted molecular weight (~458 kDa) and use a low-percentage SDS-PAGE gel with extended transfer times.
• Reported cross-reactivity with mouse and rat; validate in your specific sample system before quantitative assays.
• As a secreted ECM protein, optimize sample preparation using appropriate extraction buffers for tissue or cell culture samples.
• ELISA applications may require native or denatured antigen; pilot your coating conditions accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop sourcing for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-Tenascin-X polyclonal antibody supports extracellular matrix and cell adhesion research. Our supply network can accommodate rare-target raw-material sourcing to accelerate your diagnostic development.