Anti-Tenascin C Polyclonal Antibody for WB - P24821

Core Product Specifications and Parameters

Biological Background: Tenascin C Function and Localization

• Tenascin C (TN-C) is a large extracellular matrix glycoprotein belonging to the tenascin family, also known as cytotactin, hexabrachion, neuronectin, and glioma-associated extracellular matrix antigen. It is encoded by the TNC gene (Gene ID 3371).
• In the central nervous system, TN-C guides migrating neurons and axons during development, promotes neurite outgrowth from cortical neurons, and participates in synaptic plasticity and neuronal regeneration.
• TN-C serves as a ligand for several integrins, including alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3, and alpha-V/beta-6, mediating cell–matrix interactions.
• In tumors, TN-C stimulates angiogenesis by promoting endothelial cell elongation, migration, and sprouting. Related references: PMID:19884327
• Subcellular localization: Secreted into the extracellular space and extracellular matrix.
• Tissue specificity: Detected in fibroblasts at the protein level.
• Additional features: Contains EGF-like domains, fibronectin type-III repeats, and a C-terminal fibrinogen domain; subject to alternative splicing and phosphorylation; associated with deafness and disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 1990–2201) of human Tenascin C; for detection of endogenous full-length protein (approx. 241 kDa), consider using reducing SDS-PAGE and validate in relevant sample systems.
• Polyclonal antibodies may recognize multiple isoforms or degraded fragments; confirm specificity using appropriate controls and, if necessary, isoform-specific reagents.
• For immunohistochemistry (IHC-P) on paraffin-embedded sections, antigen retrieval may be required due to the extracellular matrix localization of TN-C.
• For immunofluorescence (IF/ICC), include permeabilization steps and optimize fixation conditions, as secreted matrix proteins can be challenging to detect.
• Cross-reactivity with mouse and rat has been reported; verify performance in your specific context, as epitope conservation may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement solution for diagnostic raw materials, including validated antibodies like this Tenascin C polyclonal antibody. Our supply network covers bulk reagents, ancillary materials, and rare target sourcing to support your extracellular matrix and oncology research projects. From concept to clinic, CamelBio bridges the gap between discovery and diagnostic application.