Anti-TELO2 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y4R8

Core Product Specifications and Parameters

Biological Background: TELO2 Function and Localization

• TELO2 (Telomere length regulation protein TEL2 homolog, also known as protein clk-2 homolog or hCLK2) is a regulator of the DNA damage response (DDR).
• It is a component of the TTT complex, which stabilizes phosphatidylinositol 3-kinase-related protein kinases (PIKKs) such as ATM, ATR, and DNA-PKcs, thereby contributing to cellular resistance against genotoxic stresses including ionizing radiation, UV, and mitomycin C.
• Together with the TTT complex and HSP90, TELO2 participates in the proper folding of newly synthesized PIKKs, ensuring their maturation and function.
• TELO2 promotes the assembly, stabilization, and activity of both mTORC1 and mTORC2 complexes, which are central regulators of cell growth, metabolism, and survival in response to nutrient and hormonal cues.
• The protein localizes to the cytoplasm, membrane, and nucleus, including chromosome telomeres, consistent with its roles in DDR and telomere maintenance.
• Post-translational modifications include acetylation, hydroxylation, phosphorylation, and ubiquitin-like conjugation, which may regulate its activity and interactions.
• Mutations in TELO2 are associated with intellectual disability and other developmental disorders, highlighting its importance in neurodevelopment.
• Additionally, TELO2 has been implicated in telomere length regulation, possibly linking genome stability and aging.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 450–550 of human TELO2) targets a region distant from known functional domains, which may help detect both modified and unmodified forms.
• For Western blotting, a band near 92 kDa is expected; pre-stained markers are recommended to confirm molecular weight, and non-specific bands may be reduced by optimizing blocking and antibody concentration.
• Polyclonal antibodies recognize multiple epitopes, offering robust signal but may require careful validation with appropriate positive and negative controls (e.g., siRNA knockdown, knockout lysates).
• Cross-reactivity is reported for human and mouse; consider species-specific verification if using in other models.
• Given TELO2's involvement in DNA damage response, treating cells with genotoxic agents (e.g., UV, ionizing radiation) may be used to study changes in TELO2 levels or localization.
• For ELISA applications, use the immunizing peptide or recombinant protein as standard to ensure quantitative performance; linearity and sensitivity should be titrated experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, supporting diagnostic manufacturers and researchers from discovery to commercialization. This anti-TELO2 polyclonal antibody serves as a critical tool for studying DNA damage repair, mTOR signaling, and telomere maintenance; we also offer optimized antibody pairs, bulk reagents, and rare target sourcing to streamline your assay development.