Anti-TEKT1 Rabbit Polyclonal Antibody for WB, ELISA - Q969V4

Core Product Specifications and Parameters

Biological Background: TEKT1 Function and Localization

• TEKT1 encodes tektin-1, a member of the tektin family of filamentous proteins that are integral components of microtubules in cilia and flagella.
• As a microtubule inner protein (MIP), TEKT1 is part of the dynein-decorated doublet microtubules (DMTs) in the axoneme of motile cilia and flagella, contributing to their structural stability.
• TEKT1 forms filamentous polymers in the walls of ciliary and flagellar microtubules, playing a critical role in axonemal architecture and function.
• Subcellular localization studies show TEKT1 in the cytoplasm and specifically associated with the axonemes of cilia and flagella.
• Predominantly expressed in testis, with additional expression in airway epithelial cells, consistent with its role in motile cilia and sperm motility. Related references: PMID:36191189
• The protein contains coiled-coil regions and is subject to ubiquitin-like conjugation (Ubl conjugation), as indicated by UniProt keywords, suggesting potential regulation by post-translational modifications.
• TEKT1 is classified under keywords including 3D-structure, cell projection, cilium, flagellum, and microtubule, highlighting its structural role in cellular motility organelles.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-300), which may help reduce cross-reactivity with other tektin family members if epitopes are less conserved in this region.
• For Western blot, expect a band around 48 kDa; consider validating in testis tissue lysates or ciliated cell lines to confirm specificity.
• For ELISA, the recombinant protein immunogen can serve as a positive control; optimize coating concentration and secondary antibody dilution experimentally.
• Cross-reactivity with mouse and rat is indicated; however, verify in the relevant species' sample systems to ensure comparable performance.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For targets involved in ciliary biology, infertility, and respiratory diseases, our catalog includes validated polyclonal antibodies, optimized monoclonal antibodies, and antibody pairs for assay development. We also offer bulk ancillary reagents and can source rare-target raw materials to support your diagnostic and research workflows.