Anti-TEK (TIE2) Rabbit Polyclonal Antibody for WB, ELISA - Q02763

Core Product Specifications and Parameters

Biological Background: TEK (Angiopoietin-1 receptor) Function and Localization

• TEK is a tyrosine-protein kinase cell-surface receptor for angiopoietins (ANGPT1, ANGPT2, ANGPT4) that critically regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion, and cytoskeletal reorganization.
• It maintains vascular quiescence by preventing leakage of pro-inflammatory plasma proteins and leukocytes, while also promoting vascular stability in resting endothelium.
• TEK signaling is context‑dependent: in quiescent vessels with tight cell‑cell contacts, ANGPT1 recruits TEK to intercellular junctions and preferentially activates the PI3K/AKT1 pathway; in migrating endothelial cells lacking adhesions, TEK localizes to focal adhesions and stimulates sprouting angiogenesis via PTK2/FAK and MAPK1/ERK2/MAPK3/ERK1 cascades.
• Modulation occurs through lower‑affinity ANGPT2, which can act as a competitive antagonist of ANGPT1, through heterodimerization with TIE1, and through proteolytic cleavage that releases a soluble extracellular decoy receptor.
• Subcellularly, TEK is found at the cell membrane, cell‑cell junctions, and focal adhesions, and is also detected in the cytoplasm, associated with the cytoskeleton, and secreted as a soluble ectodomain.
• Expression is predominantly in endothelial cells and their progenitors (angioblasts), with highest levels in placenta and lung; the soluble form is detectable in blood plasma.
• Key post‑translational modifications include ligand‑dependent autophosphorylation on tyrosine residues, glycosylation, disulfide bond formation, and ubiquitination.
• TEK is essential for embryonic angiogenesis, heart development, and postnatal hematopoiesis.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 500–750 within the cytoplasmic domain; the antibody is expected to recognize the intracellular portion of TEK. For Western blot, a band near 126 kDa should be detected under reducing conditions; consider using membrane‑enriched or whole‑cell lysates from endothelial cells.
• Cross‑reactivity with human, mouse, and rat enables studies across common model organisms. Validate species‑specific reactivity in your experimental system before use.
• For ELISA applications, pairing with a detection antibody against a non‑overlapping epitope may improve sensitivity. Optimize antigen coating and blocking conditions for your plate format.
• Proteolytic processing produces a soluble ~75 kDa extracellular fragment; this may appear in conditioned media or plasma samples. Adjust sample preparation accordingly if targeting the extracellular portion.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to high‑quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For TEK‑related angiogenesis and vascular biology studies, we offer one‑stop sourcing of anti‑TEK antibodies, recombinant angiopoietin receptors, and rare target raw materials, supporting your diagnostic development from concept to clinic.