Anti-TEK Rabbit Polyclonal Antibody for WB and ELISA - Q02763

Core Product Specifications and Parameters

Biological Background: TEK (Angiopoietin-1 Receptor) Function and Localization

• TEK (TIE2, CD202B) is a receptor tyrosine kinase that binds angiopoietins (ANGPT1, ANGPT2, ANGPT4) and plays a central role in angiogenesis and vascular stability.
• It regulates endothelial cell survival, proliferation, migration, adhesion, and cytoskeletal reorganization, and is required for normal embryonic angiogenesis and heart development.
• TEK exists as a full-length transmembrane receptor and a soluble decoy receptor generated by proteolytic processing; the soluble form is detected in blood plasma.
• It is predominantly expressed in endothelial cells and their progenitors, with high expression in placenta and lung, and lower levels in brain and kidney.
• Subcellular localization includes the cell membrane, cell junctions, focal adhesions, and cytoplasm/cytoskeleton; it is also secreted as a soluble form.
• Key post-translational modifications include ligand-induced autophosphorylation, disulfide bond formation, glycosylation, and ubiquitin conjugation.
• Mutations in TEK are associated with venous malformations and glaucoma.
• The protein contains an EGF-like domain, immunoglobulin domains, and a kinase domain.

Experimental Guidance and Technical Tips

• For Western blot, consider using endothelial cell lines (e.g., HUVEC) to validate a band near 126 kDa under reducing conditions.
• For ELISA, the immunogen fragment (aa 500-750) may capture both full-length and soluble forms; optimize coating antigen and detection conditions accordingly.
• Because TEK shares domains with other receptors, confirm specificity using knockdown controls or by cross-referencing with known expression patterns.
• The antibody cross-reacts with mouse and rat, but validate sequence homology in the immunogen region if working with these species.
• Integrin-linked signaling complexes may affect TEK detection; use appropriate lysis buffers and inhibitors to preserve phosphorylation states if interested in active receptor forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents for assay development. This anti-TEK antibody supports angiogenesis and vascular biology research programs, and our supply network can additionally source rare-target antibodies, bulk reagents, and complementary proteins to streamline your diagnostic pipeline from concept to clinic.