Anti-TEFM Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96QE5

Core Product Specifications and Parameters

Biological Background: TEFM (Transcription Elongation Factor, Mitochondrial) Function and Localization

• TEFM (Transcription Elongation Factor, Mitochondrial) is a nuclear-encoded protein that functions in mitochondrial gene expression and is encoded by the TEFM (C17orf42) gene.
• TEFM acts as a transcription elongation factor that increases the processivity of mitochondrial RNA polymerase, ensuring complete transcription of the mitochondrial genome. Related references: PMID:21278163 PMID:36823193
• It regulates the expression of oxidative phosphorylation genes, playing a crucial role in cellular energy metabolism. Related references: PMID:21278163 PMID:36823193
• The protein localizes to the mitochondrial matrix and is found in association with mitochondrial nucleoids, indicating its role in mtDNA transactions.
• Alternative splicing produces multiple isoforms, and mutations in TEFM are linked to primary mitochondrial diseases.
• The mature protein, after cleavage of the transit peptide, has a predicted molecular weight of approximately 41.7 kDa, with an observed band around 42 kDa.

Experimental Guidance and Technical Tips

• The immunogen used (amino acids 36-210) corresponds to a region of human TEFM likely representing the mature protein after transit peptide removal or a conserved functional domain. For western blot, a band at ~42 kDa is expected; consider using mitochondrial-enriched lysates for optimal detection.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), verify mitochondrial staining patterns by co-localization with mitochondrial markers. As a polyclonal antibody, cross-reactivity can be minimized by validating with knockout or knockdown samples.
• In ELISA, the antibody may be suitable for quantitative detection of TEFM; perform standard curve analyses with recombinant protein as control.
• Since the antibody cross-reacts with mouse and rat TEFM based on specification, it is potentially suitable for comparative studies across these species; however, validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive partner for diagnostic manufacturers, laboratories, and research institutes, offering one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. This TEFM polyclonal antibody is part of our extensive catalog supporting mitochondrial biology and transcription research. Beyond individual reagents, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target material sourcing—helping you accelerate assay development and production with reliable supply chains.