Anti-Tec Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P42680

Core Product Specifications and Parameters

Biological Background: Tyrosine-protein kinase Tec Function and Localization

• Tyrosine-protein kinase Tec (encoded by the TEC gene; also known as PSCTK4) is a non-receptor tyrosine kinase belonging to the Tec family of kinases, characterized by a pleckstrin homology (PH) domain, Tec homology (TH) domain, Src homology 2 and 3 (SH2 and SH3) domains, a kinase domain, and a zinc-finger motif.
• Tec functions as a signal transducer in multiple receptor-mediated pathways, regulating the actin cytoskeleton, and plays a critical role in the adaptive immune response by participating in T-cell receptor (TCR) and B-cell receptor (BCR) signaling.
• In T-cells, Tec acts redundantly with ITK to regulate development, differentiation, and function; it is required for TCR-dependent IL2 gene induction and contributes to CD28 co-stimulatory signaling by phosphorylating the adapter protein DOK1.
• In B-cells, Tec redundantly supports BTK in BCR-mediated signaling for development and activation, notably by phosphorylating STAP1, a BCR-signaling protein.
• Beyond lymphocytes, Tec is involved in mast cell cytokine production, myeloid cell growth and differentiation via CSF3 (G-CSF) signaling, and platelet activation downstream of integrin and G protein-coupled receptor pathways.
• Additionally, Tec cooperates with JAK2 to mediate cytokine-driven FOS transcription, phosphorylates GRB10, regulates the unconventional secretion of FGF2, and may participate in hepatocyte proliferation and osteoclast differentiation.
• Subcellularly, Tec localizes to the cytoplasm, cell membrane, and cytoskeleton, enabling dynamic signaling complex assembly.
• Expression of TEC is widespread in hematopoietic lineages, including myeloid, B-cell, and T-cell lines, consistent with its broad immunoregulatory functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-280 of human Tec, which encompasses the N-terminal PH, TH, and SH3 domains. Consider that this region may be conserved across species, supporting the reported cross-reactivity with mouse and rat.
• For Western blotting, expect a band at approximately 74 kDa in Tec-expressing cell lines such as Jurkat (T-cell) or Ramos (B-cell); validate detection in relevant sample systems and include appropriate positive and negative controls.
• In immunofluorescence/immunocytochemistry (IF/ICC), Tec is expected to show cytoplasmic and membrane localization; optimize fixation and permeabilization conditions to preserve epitope accessibility.
• For immunohistochemistry (IHC-P), antigen retrieval and antibody titration should be empirically determined, as performance can vary with tissue type and processing.
• This polyclonal antibody may be used in ELISA; consider paired-antibody development and validate sensitivity and specificity using recombinant Tec or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering manufacturers, laboratories, and research institutes streamlined access to antibodies, antigens, and technical support from concept to clinic. This anti-Tec polyclonal antibody supports investigation into tyrosine kinase signaling and immune cell development; our portfolio includes validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, ensuring continuity for diagnostic and research workflows.