Anti-Tec Monoclonal Antibody for WB and ELISA - P42680

Core Product Specifications and Parameters

Biological Background: Tec Function and Localization

• Tec is a non-receptor tyrosine kinase and a member of the Tec kinase family, which also includes BTK, ITK, RLK, and BMX.
• It plays a key role in signaling from multiple receptors, particularly in the adaptive immune system, regulating T-cell and B-cell development, differentiation, and function.
• In T-cells, Tec is required for TCR-dependent IL2 gene induction and negatively regulates IL2RA expression following TCR cross-linking.
• In B-cells, Tec acts redundantly with BTK in BCR signaling, phosphorylating STAP1 to support B-cell development and activation.
• Tec contributes to mast cell cytokine production, myeloid cell differentiation via CSF3, and platelet signaling downstream of integrin activation.
• It localizes to the cytoplasm, cell membrane, and cytoskeleton, consistent with its role in actin cytoskeleton regulation.
• Tec is widely expressed in hematopoietic lineages, including myeloid, B-cell, and T-cell lines.
• The protein features SH2, SH3, and zinc-finger domains, an ATP-binding site, and kinase activity, classifying it as a transferase.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1–280 of human Tec (N-terminal region); this antibody may detect total Tec, but validate for specific isoforms or cleavage products in your system.
• For Western blot, the predicted molecular weight is 74 kDa; use reducing conditions and confirm expression in human, mouse, or rat hematopoietic cell lysates.
• ELISA application is indicated; optimize antibody coating and detection conditions for your specific assay format.
• Cross-reactivity is reported for human, mouse, and rat; nonetheless, verify in your target tissue and species, as expression varies among hematopoietic lineages.
• No quantitative performance data are provided; titrate antibody for each application and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a seamless sourcing experience for IVD raw materials, connecting diagnostic manufacturers and research labs with validated antibodies like this anti-Tec monoclonal antibody, which supports investigations into immune signaling and hematologic disorders. Backed by robust supply capabilities—including bulk monoclonals, ancillary reagents, and rare-target procurement—we help accelerate your projects from concept to clinical readiness.