Anti-TEAD4 Rabbit Polyclonal Antibody for WB, ELISA - Q15561

Core Product Specifications and Parameters

Biological Background: TEAD4 Function and Localization

• Transcriptional enhancer factor TEF-3 (TEAD4), also designated TEA domain family member 4, is a transcription factor encoded by the TEAD4 gene (GeneID: 7004) and is subject to alternative splicing.
• TEAD4 is a core component of the Hippo signaling pathway that controls organ size and suppresses tumorigenesis by restricting cell proliferation and promoting apoptosis.
• Within the Hippo cascade, MST1/2 kinases (with SAV1) phosphorylate and activate LATS1/2 (with MOB1), which in turn phosphorylate YAP1 and WWTR1/TAZ; TEAD4 then mediates the transcriptional output of YAP1/TAZ to regulate proliferation, migration, and epithelial-mesenchymal transition (EMT).
• It binds specifically and non-cooperatively to the Sph and GT-IIC enhansons (5'-GTGGAATGT-3') and to the M-CAT motif, thereby activating target gene transcription.
• The protein localizes predominantly to the nucleus, consistent with its role as a DNA-binding transcriptional activator.
• TEAD4 is preferentially expressed in skeletal muscle, with lower levels detected in pancreas, placenta, and heart.
• The mature protein has a calculated molecular weight of approximately 48 kDa (UniProt: 48,329 Da; observed ~48 kDa on SDS-PAGE).

Experimental Guidance and Technical Tips

• For Western blotting, consider preparing whole-cell lysates from human skeletal muscle or relevant cell lines that express TEAD4 endogenously to serve as positive controls.
• Given the predicted molecular weight of ~48 kDa, standard SDS-PAGE conditions and a 10% gel are suitable; validate the antibody’s optimal dilution in your sample system.
• For ELISA, the immunogen spans amino acids 110–250, a region that may present accessible epitopes; consider using a recombinant fragment or peptide coating for indirect ELISA protocols.
• Cross‑reactivity with mouse and rat TEAD4 has been confirmed by the supplier; confirm reactivity in your species of interest using appropriate tissue lysates or recombinant protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one‑stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and research laboratories with validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. The TEAD4 rabbit polyclonal antibody supports research into the Hippo signaling pathway and transcription‑related disorders, complementing our portfolio of high‑quality reagents designed to accelerate your assay development from concept to clinic.