Anti-TEAD3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99594

Core Product Specifications and Parameters

Biological Background: TEAD3 Function and Localization

• TEAD3, also designated Transcriptional enhancer factor TEF-5, DTEF-1, or TEAD-3, is a member of the TEA domain (TEAD) family of transcription factors, encoded by the TEAD3 gene (GeneID: 7005) in humans.
• It functions as a downstream effector of the Hippo signaling pathway, mediating gene expression regulation upon YAP1 and WWTR1/TAZ co-activation, thereby controlling cell proliferation, migration, and epithelial-mesenchymal transition (EMT).
• TEAD3 directly binds to specific DNA enhancer elements, such as those in the human chorionic somatomammotropin-B gene enhancer, to regulate transcription.
• The protein localizes predominantly to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Preferentially expressed in the placenta, TEAD3 may play tissue-specific roles in development and growth regulation.
• Post-translational modifications include acetylation and phosphorylation, which may modulate its activity and protein stability.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fragment spanning amino acids 130-330 of human TEAD3; this region may help detect total TEAD3 across human, mouse, and rat samples in WB applications.
• For western blotting, consider optimizing protein loading and antibody dilution to detect the predicted 49 kDa band; include appropriate controls to confirm specificity.
• In IHC and IF/ICC, validate the antibody with known positive tissues (e.g., placenta) and expected nuclear localization due to TEAD3's subcellular distribution.
• For ELISA, the antibody may serve as a capture or detection reagent; pairing with matched antibody sets is recommended for sandwich ELISA workflows.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a one-stop sourcing platform for IVD raw materials, including validated antibodies like TEAD3 rabbit polyclonal antibodies for Hippo signaling and transcription factor studies. Our portfolio covers antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents, with support for rare target raw-material sourcing across your assay development stages. Contact us to streamline your supply chain from concept to clinic.