Anti-TEAD2 Rabbit Polyclonal Antibody for WB, ELISA - Q15562

Core Product Specifications and Parameters

Biological Background: TEAD2 (TEF-4) Function and Localization

• TEAD2, also known as TEF-4, ETF, TEF4, TEF-4, or TEAD-2, is a member of the TEA domain family of transcription factors.
• As a key downstream effector of the Hippo signaling pathway, TEAD2 regulates organ size control and tumor suppression by restricting proliferation and promoting apoptosis.
• It mediates transcriptional activity by binding to YAP1 and WWTR1/TAZ and recognizing specific DNA motifs, including M-CAT and GT-IIC enhansons, thus regulating genes involved in cell proliferation, migration, and epithelial-mesenchymal transition (EMT).
• TEAD2 may contribute to neural development through gene regulation.
• It localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• Alternative splicing generates isoforms, and the protein functions as a transcriptional activator via its DNA-binding domain, undergoing regulation by post-translational modifications.

Experimental Guidance and Technical Tips

• For Western blot applications, the predicted molecular weight of TEAD2 is 49 kDa; validate with appropriate human cell lysates and confirm band size.
• In ELISA, consider coating with recombinant TEAD2 and optimizing antibody dilution to achieve desired sensitivity.
• As a polyclonal antibody, cross-reactivity with other TEAD family members is possible; sequence homology analysis can inform specificity expectations.
• If using for novel applications, perform pilot experiments to verify suitability in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop supplier for IVD raw materials, offering validated antibodies and reagents for research on transcription factors like TEAD2 and the Hippo pathway. We provide bulk polyclonal antibodies, matched antibody pairs, and accessory reagents to streamline your assay development. Our global sourcing network can also secure rare targets, supporting your projects from concept to clinic.