Anti-TEAD1/2/3/4 Rabbit Monoclonal Antibody for WB, ELISA - P28347 / Q15561 / Q15562 / Q99594

Core Product Specifications and Parameters

Biological Background: TEAD1 Function and Localization

• TEAD1 (TEF-1, NTEF-1) is a transcription factor and a key effector of the Hippo signaling pathway, which controls organ size and suppresses tumorigenesis by regulating cell proliferation and apoptosis.
• It mediates the transcriptional output of YAP1 and WWTR1/TAZ co-activators, driving expression of genes involved in cell growth, migration, and epithelial-mesenchymal transition (EMT).
• TEAD1 binds specifically to MCAT, SPH, and GT-IIC enhancer elements (consensus: 5'-GTGGAATGT-3'), activating transcription in a cell-type-specific manner.
• It is essential for cardiac development and is preferentially expressed in skeletal muscle, with lower levels in pancreas, placenta, and heart.
• The protein localizes to the nucleus and undergoes post-translational modifications including acetylation and phosphorylation, which modulate its activity.
• TEAD1 belongs to the TEA domain (TEAD) family of transcription factors, which in humans comprises four members (TEAD1-4) that share conserved DNA-binding domains.
• Alternatively spliced isoforms and natural variants of TEAD1 have been described, some linked to human diseases.

Experimental Guidance and Technical Tips

• This antibody was generated against an immunogen corresponding to the C-terminal region (aa 174-263) of human TEAD4, a region that may share epitope similarity across TEAD family members, consistent with its pan-TEAD reactivity.
• For Western blotting, consider using reducing conditions and validate the observed band sizes (expected ~48-49 kDa) in relevant human cell lysates; note that multiple TEAD isoforms may migrate closely.
• For ELISA, ensure proper coating and titration of the antibody, and validate specificity using recombinant TEAD proteins or transfected cell lysates.
• Since TEAD proteins are regulated by the Hippo pathway, consider stimulus-dependent changes (e.g., serum concentration, cell density) when interpreting expression levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to validated IVD raw materials, offering antibodies like this pan-TEAD monoclonal antibody to support Hippo pathway and cancer research. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and the sourcing of rare-target raw materials, helping you transition seamlessly from concept to clinical application.