Anti-TEAD1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA, ChIP - P28347

Core Product Specifications and Parameters

Biological Background: TEAD1 Function and Localization

• TEAD1 (Transcriptional Enhancer Factor TEF-1, NTEF-1, TCF-13) is a member of the TEA domain family of transcription factors and exists as multiple isoforms due to alternative splicing.
• It functions as a key downstream effector of the Hippo signaling pathway, mediating the transcriptional activity of YAP1 and WWTR1/TAZ to regulate cell proliferation, migration, and epithelial-mesenchymal transition (EMT).
• TEAD1 specifically binds to the SPH and GT-IIC enhansons (5'-GTGGAATGT-3') and the M-CAT motif, activating transcription in a cell-type-specific manner through a limiting transcriptional intermediary factor (TIF).
• It plays a critical role in cardiac development by regulating genes essential for heart morphogenesis.
• The protein is localized to the nucleus, consistent with its function as a transcription factor.
• Post-translational modifications include acetylation and phosphorylation, which modulate its activity and interactions.
• TEAD1 is preferentially expressed in skeletal muscle, with lower expression in the pancreas, placenta, and heart.

Experimental Guidance and Technical Tips

• As a polyclonal antibody raised against a synthetic peptide, this product may be suitable for detecting multiple isoforms of TEAD1.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider testing fixation and permeabilization conditions to optimize nuclear staining.
• The reported cross-reactivity with mouse and rat suggests the immunogen sequence is conserved; however, validate detection in your experimental system.
• For ChIP applications, polyclonal antibodies often provide robust enrichment due to recognition of multiple epitopes; include appropriate IgG controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with reliable access to validated IVD raw materials, including optimized antibodies like this anti-TEAD1 polyclonal antibody for Hippo pathway and transcription research. Our comprehensive portfolio spans antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents, complemented by tailored technical services to streamline your assay development.