Anti-TEAD1 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA, ChIP - P28347

Core Product Specifications and Parameters

Biological Background: TEAD1 Function and Localization

• TEAD1 is a transcription factor and a key component of the Hippo signaling pathway, which controls organ size and suppresses tumors by restricting proliferation and promoting apoptosis.
• It mediates gene expression by associating with YAP1 and WWTR1 (TAZ), regulating cell proliferation, migration, and epithelial-mesenchymal transition (EMT).
• TEAD1 binds to the SPH and GT-IIC enhansons (5'-GTGGAATGT-3') and activates transcription in a cell-specific manner, interacting with a limiting cell-specific transcriptional intermediary factor (TIF).
• Involved in cardiac development and binds to the M-CAT motif.
• Subcellular location: Nucleus.
• Preferentially expressed in skeletal muscle, with lower levels in pancreas, placenta, and heart.
• The protein undergoes acetylation and phosphorylation and is subject to alternative splicing.
• Keywords: 3D-structure, Acetylation, Activator, Alternative splicing, Disease variant, DNA-binding, Nucleus, Phosphoprotein, Transcription, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 135–215) corresponds to a region critical for DNA binding; the polyclonal antibody likely includes antibodies targeting epitopes involved in TEAD1-DNA interaction, potentially useful for ChIP.
• In WB, anticipate a band near 48 kDa; however, due to isoforms and PTMs, actual migration may differ; validate using positive controls like skeletal muscle lysate.
• For IP and ChIP, the polyclonal nature may improve capture efficiency; consider optimizing antibody concentration and crosslinking conditions for ChIP analysis of TEAD1 binding sites.
• For IHC-P and IF/ICC, use skeletal muscle tissue as a positive control; note that antigen retrieval and permeabilization steps may be required for nuclear detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with high-quality reagents. For transcription factor and pathway studies such as those involving TEAD1 and the Hippo signaling cascade, we offer a range of optimized antibodies, recombinant proteins, and ancillary reagents. Rely on us for efficient procurement, from validated antibody pairs and bulk polyclonal antibodies to rare-target raw materials, accelerating your assay development from concept to clinic.