Anti-TdT Rabbit Polyclonal Antibody for WB, ELISA - P04053

Core Product Specifications and Parameters

Biological Background: TdT Function and Localization

• DNA nucleotidylexotransferase (TdT), also known as terminal addition enzyme or terminal deoxynucleotidyltransferase, is a template-independent DNA polymerase encoded by the DNTT gene.
• It catalyzes the random addition of deoxynucleoside triphosphates to the 3'-hydroxyl ends of DNA fragments, playing a crucial role in V(D)J recombination by generating N-region diversity in immunoglobulin and T-cell receptor genes during B- and T-cell maturation.
• TdT is a member of the DNA polymerase X family and functions as a nucleotidyltransferase, requiring divalent metal ions such as magnesium as cofactors.
• The protein contains several metal-binding sites essential for its catalytic activity.
• It is predominantly localized in the nucleus, consistent with its role in DNA modification during lymphocyte development.
• Alternative splicing of the DNTT transcript produces multiple isoforms, and the protein undergoes phosphorylation, as indicated by proteomic studies.
• Structural studies (3D-structure keyword) have provided insights into its mechanism, and it is classified as a reference proteome component.
• Its function is associated with lymphoid tissues where V(D)J recombination occurs.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 260-509 of human TdT, a region that may help target specific isoforms; verify performance in your specific sample system.
• For Western blot (WB) applications, a band around 59kDa/58.5kDa (depending on isoform) is expected; consider using appropriate loading controls.
• This polyclonal antibody is suitable for ELISA; titrate the antibody to optimize sensitivity and specificity for your assay format.
• Cross-reactivity with mouse and rat TdT is indicated; validate in relevant murine or rat lymphoid tissues if using these species.
• Given TdT's nuclear localization, ensure proper nuclear extraction or permeabilization in WB protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs streamlined access to critical IVD raw materials, including validated antibodies for immunology and oncology targets. For TdT research, we can supply optimized monoclonal and polyclonal antibody pairs, bulk ancillary reagents, and assist with sourcing rare-target materials to support your assay development from concept to commercialization.