Anti-TdT Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P04053

Core Product Specifications and Parameters

Biological Background: TdT Function and Localization

• Terminal deoxynucleotidyltransferase (TdT), encoded by the DNTT gene, is a template-independent DNA polymerase that catalyzes the random addition of deoxynucleoside triphosphates to the 3'-OH end of DNA initiators.
• TdT plays a critical role in V(D)J recombination by adding non-templated (N) nucleotides at the junctions of immunoglobulin heavy chain and T-cell receptor gene segments during B- and T-lymphocyte maturation.
• The enzyme requires divalent metal ions, such as magnesium, for its catalytic activity, as indicated by the "Magnesium" and "Metal-binding" UniProt keywords.
• TdT is localized predominantly in the nucleus, where it associates with the recombination machinery to generate junctional diversity.
• Post‑translational modifications include phosphorylation, as annotated by the "Phosphoprotein" keyword, which may regulate enzyme activity or protein interactions.
• Alternative splicing of the DNTT transcript produces multiple isoforms, as noted by the "Alternative splicing" keyword, potentially influencing functional specialization.
• The protein’s three‑dimensional structure has been resolved ("3D-structure" keyword), providing insights into the molecular mechanism of template‑independent nucleotide addition.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 10–124 of human TdT, a region likely to generate antibodies that recognize the native protein; consider verifying reactivity with endogenous TdT in relevant lymphocyte models.
• For western blotting, a band at approximately 58–59 kDa is expected; validate detection in nuclear extracts from thymus or leukemia cell lines known to express TdT.
• In immunofluorescence or immunohistochemistry, anticipate nuclear staining; include appropriate permeabilization steps and confirm signal specificity using TdT‑negative samples or knockout controls.
• The antibody may be suitable for ELISA‑based quantitation of recombinant or purified TdT; optimize the coating antigen and detection format for your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement experience for IVD manufacturers and researchers seeking high‑quality TdT antibodies and associated raw materials. With validated monoclonal antibodies, bulk reagent supply, and expertise in rare‑target sourcing, we support the development of diagnostics and assays in immunology and oncology. Reach out to explore our full portfolio of DNA polymerase antibodies and ancillary reagents for your next project.