Anti-TDRD3 Rabbit Polyclonal Antibody for WB and ELISA - Q9H7E2

Core Product Specifications and Parameters

Biological Background: TDRD3 Function and Localization

• TDRD3 is a scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins, mediating interactions with Arg/Gly-rich motifs (GAR). Related references: PMID:15955813
• In the nucleus, TDRD3 acts as a transcriptional coactivator by binding asymmetrically dimethylated histone tails H3R17me2a and H4R3me2a, recruiting proteins to arginine-methylated loci. Related references: PMID:21172665
• In the cytoplasm, TDRD3 functions as an antiviral factor by participating in the assembly of stress granules together with G3BP1. Related references: PMID:35085371
• TDRD3 is widely expressed, detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• The protein localizes to both the cytoplasm and the nucleus.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond, Ubl conjugation), as indicated by UniProt keywords.
• Multiple isoforms are produced by alternative splicing.
• TDRD3 belongs to the Tudor domain-containing protein family, characterized by a Tudor domain that recognizes methylated arginines.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–220) of human TDRD3; consider this when designing complementary reagents.
• For western blot, the predicted molecular weight is approximately 73 kDa; multiple bands may appear due to alternative splicing variants—validate in the relevant human sample system.
• Direct and sandwich ELISA formats can be explored; this antibody has been used in ELISA, but optimal conditions should be determined empirically.
• Human specificity is reported; cross-reactivity with other species has not been tested and should be verified if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. For TDRD3 research, we can provide customized reagents to support assay development and translational studies, serving as a reliable partner from concept to clinic.