Anti-TDRD1 Polyclonal Antibody for WB, ELISA - Q9BXT4

Core Product Specifications and Parameters

Biological Background: TDRD1 Function and Localization

• TDRD1, also known as cancer/testis antigen 41.1 (CT41.1), is a tudor domain-containing protein involved in germ cell development.
• It plays a central role in spermatogenesis by repressing transposable elements through the piRNA pathway, a process essential for maintaining genome integrity in the germline.
• TDRD1 facilitates the entry of correct transcripts into the piRNA pool and restricts aberrant cellular transcripts, thereby ensuring piRNA biogenesis fidelity.
• It is required for the localization of Piwi proteins to the meiotic nuage, a cytoplasmic germ cell structure critical for piRNA function.
• Subcellularly, TDRD1 is found in the cytoplasm and localizes specifically to the meiotic nuage.
• Expression is predominantly testis- and ovary-specific, with aberrant expression detected in several cancers, consistent with its classification as a cancer/testis antigen.
• The protein contains Tudor and zinc-finger domains, suggesting metal-binding properties, and is involved in developmental processes including meiosis and spermatogenesis.
• Alternative splicing generates multiple isoforms of TDRD1, adding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment spanning amino acids 25–125; for Western blot, consider using lysates from human testis tissue or cancer cell lines known to express TDRD1.
• Given the polyclonal nature of this antibody, it may recognize multiple bands if isoforms or degradation products are present; validate the observed band size against the predicted ~132 kDa in the relevant sample system.
• For ELISA applications, the recombinant immunogen or a corresponding peptide can be employed as a standard or coating antigen; optimal antibody dilution should be determined empirically.
• Cross-reactivity with mouse has been observed, making this antibody suitable for studies in mouse models; nevertheless, verify performance in your specific assay conditions.
• Due to the low expression of TDRD1 in normal somatic tissues, careful selection of positive controls (e.g., testis lysate, transfected cells) is essential to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with premium IVD raw materials, offering a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This TDRD1 polyclonal antibody supports reproductive biology and cancer research applications, providing a reliable tool for piRNA pathway studies. Leverage CamelBio’s sourcing expertise to secure high-quality, rare-target reagents and streamline your assay development from concept to clinic.