Anti-TDP2 Rabbit Polyclonal Antibody (KO Validated) for WB, ELISA - O95551

Core Product Specifications and Parameters

Biological Background: TDP2 Function and Localization

• TDP2 is a DNA repair hydrolase that specifically removes 5'-tyrosyl adducts from DNA, resolving topoisomerase 2 (TOP2)-DNA dead-end complexes and generating clean DNA double-strand breaks with 5'-phosphate termini suitable for ligation, thereby protecting neuronal gene transcription from abortive TOP2 activity. Related references: PMID:27060144 PMID:27099339

• Functions in the cellular stress response by regulating ribosome biogenesis and participates in non-canonical TGF-beta signaling as an adaptor, promoting TRAF6-TAK1 complex ubiquitination and activation.

• Also possesses weak 3'-tyrosyl DNA phosphodiesterase activity; represents the major, if not sole, 5'-tyrosyl DNA phosphodiesterase in cells, and hydrolyzes 5'-phosphoglycolates at radiation-induced DNA break ends.

• During picornavirus infection, acts as a 5'-tyrosyl RNA phosphodiesterase (VPg unlinkase) to cleave the viral VPg protein from genomic RNA, a step critical for viral replication.

• Localizes to multiple subcellular compartments including the nucleus, nucleolus, PML bodies, and cytoplasm, consistent with its diverse roles in DNA repair and signaling.

• Broadly expressed across tissues, with notably high levels in the brain, including the frontal and occipital lobes, hippocampus, striatum, and cerebellum. Related references: PMID:10764746 PMID:24658003

• Also known as ETS1-associated protein 2 (EAP2), TRAF and TNF receptor-associated protein (TTRAP), and VPg unlinkase; undergoes post-translational acetylation and phosphorylation, and is linked to neurodevelopmental disorders such as intellectual disability and spinocerebellar ataxia.

Experimental Guidance and Technical Tips

• The immunogen used for this polyclonal antibody corresponds to the N-terminal region (aa 1-200) of human TDP2; this region is highly conserved and suitable for detecting both full-length and potentially cleaved isoforms.
• Western blot (WB) analysis can be performed using standard protocols; the predicted molecular weight of TDP2 is ~41 kDa. Consider using a KO-validated sample as a negative control to confirm band specificity.
• ELISA applications may require coating with recombinant TDP2 protein or peptide; ensure proper blocking and antibody titration for optimal signal-to-noise ratio.
• For mouse samples, cross-reactivity is expected due to high sequence homology; however, validate the antibody in the specific tissue or cell line of interest to rule out non-specific binding.
• This antibody is shipped on ice bags; upon receipt, aliquot and store at -20°C or -80°C for long-term stability; avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a seamless sourcing experience for IVD manufacturers, laboratories, and research institutes, offering validated TDP2 antibodies alongside a full suite of ancillary reagents and custom services. Covering DNA repair and kinase signaling targets, our portfolio includes optimized monoclonal/polyclonal antibodies, bulk antigens, and rare-target raw materials. Partner with us to accelerate your diagnostic development with reliable, one-stop procurement from concept to clinic.