Anti-TDP-43/TARDBP Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13148

Core Product Specifications and Parameters

Biological Background: TDP-43/TARDBP Function and Localization

• TAR DNA-binding protein 43 (TDP-43) is encoded by the TARDBP gene and belongs to the hnRNP family of RNA-binding proteins.
• It preferentially binds GU-rich RNA sequences via its two RRM domains, regulating pre-mRNA splicing, mRNA stability, and transport. Related references: PMID:23519609, PMID:24240615, PMID:24464995
• TDP-43 is critical for the alternative splicing of transcripts involved in neuronal survival and neurodegeneration. Related references: PMID:21358640, PMID:29438978
• Mediates mRNA deadenylation and decay by recruiting the CNOT7 deadenylase to 3'UTRs. Related references: PMID:30520513
• Under oxidative stress, translocates to cytoplasmic stress granules and associates with stalled ribosomes to promote cell survival. Related references: PMID:19765185, PMID:23398327
• Regulates circadian clock periodicity by stabilizing CRY1/CRY2 proteins in an FBXL3-dependent manner. Related references: PMID:27123980
• Localizes predominantly to the nucleus but also to the cytoplasm, stress granules, and mitochondria.
• Ubiquitously expressed with enrichment in pancreas, placenta, lung, genital tract, and spleen.

Experimental Guidance and Technical Tips

• For Western blotting, empirically determine the optimal primary antibody dilution; expect a specific band near 45 kDa corresponding to TDP-43.
• When performing IHC-P or IF/ICC, optimize antigen retrieval and permeabilization protocols due to the protein's nuclear and cytoplasmic localization.
• Validate cross-reactivity in mouse and rat tissues if these species are used, as indicated by the supplier's cross-reactivity data.
• The immunogen (N-terminal 1-100 amino acids) may recognize both full-length and N-terminal isoforms; consider this in isoform-specific experiments.
• ELISA can be employed for quantification; ensure proper coating concentrations and blocking conditions are established.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material procurement, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For research targeting TDP-43 in RNA biology and neurodegeneration, this anti-TDP-43 polyclonal antibody provides a reliable reagent for multiple immunoassay formats. We support diagnostic developers and labs from concept to clinic with one-stop access to rare target raw materials and tailored sourcing solutions.