Anti-TDO2 Polyclonal Antibody for WB, IF/ICC, ELISA - P48775

Core Product Specifications and Parameters

Biological Background: TDO2 Function and Localization

• TDO2 encodes tryptophan 2,3-dioxygenase (also known as TO, TRPO, tryptophan pyrrolase), a heme-containing enzyme.
• It functions as an oxidoreductase that catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan degradation.
• The enzyme incorporates molecular oxygen into L-tryptophan, cleaving the pyrrole ring to produce N-formyl-L-kynurenine.
• TDO2 activity requires the heme cofactor and iron for proper function.
• The protein is classified under the tryptophan catabolism pathway, playing a central role in regulating systemic tryptophan levels.
• Variations in TDO2 have been associated with disease states (keyword: disease variant), though specific mutations are not detailed in the provided record.
• Additional annotations include its resolved 3D structure and identification via proteomics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (aa 100–200); verify epitope conservation across species and isoforms if cross-reactivity is critical.
• For Western blot, the expected molecular weight is approximately 48 kDa; validate detection in the relevant lysate and tissue type.
• Immunofluorescence/ICC may require optimization of fixation and permeabilization conditions; include appropriate controls.
• In ELISA, consider checkerboard titration to determine optimal coating antigen and antibody concentrations.
• As a polyclonal antibody, lot-to-lot variability may occur; it is advisable to titrate each new lot for the intended application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research communities with integrated access to IVD raw materials, including validated antibodies for metabolic and oncology research. Our anti-TDO2 polyclonal antibody supports studies on the kynurenine pathway and tryptophan metabolism. Beyond this product, we offer bulk sourcing of matched antibody pairs, monoclonal alternatives, and ancillary reagents to streamline your assay development workflows.