Anti-TDO2 Polyclonal Antibody for WB, IF/ICC, ELISA - P48775

Core Product Specifications and Parameters

Biological Background: TDO2 Function and Localization

• TDO2 encodes tryptophan 2,3-dioxygenase, a heme-dependent enzyme that catalyzes the first and rate-limiting step in the kynurenine pathway, converting L-tryptophan to N-formyl-L-kynurenine.
• Functions as an oxidoreductase, cleaving the pyrrole ring of L-tryptophan using molecular oxygen, with heme and iron as essential cofactors.
• Also known as tryptophan oxygenase (TO), tryptophan pyrrolase (TRPO), and tryptophanase, reflecting its role in tryptophan catabolism.
• Listed as a reference proteome component for Homo sapiens, with evidence at the protein level (Proteomics identification).
• Genetic variations in TDO2 are linked to disease phenotypes, as indicated by the Disease variant keyword.
• Plays a key role in tryptophan homeostasis and has been implicated in immune regulation and neurotransmitter metabolism via the kynurenine pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 100–200 of human TDO2; for optimal results, ensure this region is conserved in your target species and isoforms.
• Validated for Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA; consider titrating the antibody and including appropriate positive/negative controls for each application.
• Predicted to detect endogenous TDO2 at approximately 48 kDa; use a suitable molecular weight marker and confirm band specificity with blocking peptides or knockdown/knockout controls if needed.
• Cross-reacts with human, mouse, and rat TDO2; for other species, verify sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support diagnostic development from concept to clinic. This anti-TDO2 polyclonal antibody is well-suited for research on tryptophan metabolism and related disorders, and our team can assist with rare target raw-material sourcing and technical consultation to meet your specific assay requirements.