Anti-TDGF1/CRIPTO Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P13385

Core Product Specifications and Parameters

Biological Background: TDGF1/CRIPTO Function and Localization

• Protein Cripto (Teratocarcinoma-derived growth factor 1), encoded by the TDGF1/CRIPTO gene, is a GPI-anchored cell membrane protein belonging to the EGF-CFC family. It is also known as CR, CR-1, CRGF, or CRIPTO.
• Acts as a coreceptor for Nodal, a member of the TGF-beta superfamily. Cell-associated CRIPTO facilitates Nodal signaling in cis, while shedding by TMEM8A generates a soluble form that can function in trans on neighboring cells.
  Related references:
  PMID:27881714
• Plays a role in early embryonic development, particularly in the determination of epiblastic cells that give rise to the mesoderm.
  Related references:
  PMID:11909953
• Subcellular localization includes the cell membrane and secretion into the extracellular space, consistent with its GPI anchor and shedding.
• Preferentially expressed in gastric and colorectal carcinomas compared to normal tissues, and also detected in breast and lung cancers.
• Features an EGF-like domain, multiple disulfide bonds, and glycosylation sites. It is synthesized with a signal peptide and anchored to the membrane via a GPI moiety.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 31-150 of human TDGF1, which excludes the signal peptide (residues 1-30) and includes the EGF-like domain. This design likely enhances specificity for mature and processed forms of the protein.
• For Western blot (WB), a band around 21 kDa may be observed, but post-translational modifications such as glycosylation and GPI-anchor addition can result in higher molecular weight species. Validate detection in relevant cancer cell lines (e.g., gastric, colorectal, breast, lung).
• In immunohistochemistry (IHC-P), membrane and/or cytoplasmic staining is expected. Ensure proper antigen retrieval and consider using cancer tissue sections with known CRIPTO overexpression.
• The antibody shows cross-reactivity with mouse and rat TDGF1, making it suitable for studies in rodent models; however, validate species-specific reactivity in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner, providing diagnostic manufacturers and research laboratories with high-quality antibodies, antigens, and ancillary reagents from discovery to commercial scale. This anti-TDGF1 polyclonal antibody is part of our oncology and developmental biology portfolio, and CamelBio can further supply validated antibody pairs, blocking buffers, and bulk-order custom formulations to accelerate your assay development. Whether you need rare target sourcing or large-volume production, our integrated supply chain bridges your R&D needs from concept to clinic.