Anti-TCTP/TPT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P13693

Core Product Specifications and Parameters

Biological Background: Translationally-controlled tumor protein (TCTP/TPT1) Function and Localization

• Translationally-controlled tumor protein (TCTP), also known as fortilin, histamine-releasing factor (HRF), and p23, is encoded by the TPT1 gene.
• TCTP is involved in calcium binding and microtubule stabilization, supporting cellular structural integrity and signaling (PubMed:12167714, PubMed:15162379, PubMed:15958728). Related references: PMID:12167714 PMID:15162379 PMID:15958728
• It acts as a negative regulator of TSC22D1-mediated apoptosis by interacting with and destabilizing the TSC22D1 protein (PubMed:18325344). Related references: PMID:18325344
• The protein is predominantly localized in the cytoplasm.
• TCTP is expressed in a wide range of healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas, but is notably absent in kidney and renal cell carcinoma. It is also expressed in placenta and prostate.
• Post-translational modifications include phosphorylation; alternative splicing produces multiple isoforms. The protein has a known 3D structure and is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant human TCTP/TPT1 (aa 1-172), which may enable recognition of both native and denatured protein conformations.
• For Western blot (WB), consider using whole-cell lysates from human or mouse cell lines; the predicted molecular weight is ~20 kDa.
• For immunofluorescence (IF/ICC), fix and permeabilize cells appropriately; anticipate cytoplasmic staining based on the reported subcellular localization.
• In ELISA applications, the polyclonal nature may provide broad epitope coverage; consider pairing with a specific monoclonal antibody if developing a sandwich assay.
• Validate detection in the relevant sample system, as expression levels can vary among tissue types and absence in kidney should be noted.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline diagnostic development. Our TCTP/TPT1 polyclonal antibody supports research into apoptosis, calcium signaling, and tumor biology, and is readily available for procurement. From rare target sourcing to full-scale supply, we bridge the gap from concept to clinic with reliable one-stop service.