Anti-TCTP/TPT1 Rabbit Polyclonal Antibody for WB and ELISA - P13693

Core Product Specifications and Parameters

Biological Background: TCTP/TPT1 Function and Localization

• TCTP/TPT1, encoded by the TPT1 gene, is also known as Translationally-controlled tumor protein, Fortilin, Histamine-releasing factor (HRF), and p23.
• It plays a role in calcium binding and microtubule stabilization, contributing to cytoskeletal dynamics and cellular integrity. Related references: PMID:12167714, PMID:15162379, PMID:15958728
• TCTP acts as a negative regulator of apoptosis by interacting with and destabilizing TSC22D1 protein, thereby inhibiting TSC22D1-mediated cell death. Related references: PMID:18325344
• The protein is predominantly localized in the cytoplasm.
• It is widely expressed in healthy and tumoral cells, including erythrocytes, hepatocytes, macrophages, and various cancer types, but absent in kidney and renal cell carcinoma. Expressed in placenta and prostate.
• TCTP is subject to phosphorylation and alternative splicing, as indicated by proteomics and 3D-structure studies.
• Its molecular weight is approximately 20 kDa (calculated 19.6 kDa), and it belongs to the TPT1 family.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human TCTP sequence (amino acids 1-172), which may facilitate recognition of native and recombinant TCTP proteins across species.
• For Western blot (WB) experiments, consider using denaturing and reducing conditions, as the predicted/observed molecular weight is ~20 kDa.
• In ELISA applications, the antibody may be suitable for both direct and sandwich formats; cross-reactivity with mouse and rat TCTP should be validated in the relevant sample system.
• Given TCTP's ubiquitous expression, appropriate positive and negative control lysates (e.g., placenta vs. kidney) can help ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering reliable access to validated antibodies like this anti-TCTP/TPT1 polyclonal antibody. With expertise in calcium-binding and apoptosis-related targets, we provide bulk ancillary reagents, optimized antibody panels, and custom sourcing for rare or emerging biomarkers. Let CamelBio streamline your transition from concept to clinic with quality-assured, one-stop supply solutions.