Anti-TCTP/TPT1 Monoclonal Antibody for WB, IP, IHC-P, ELISA - P13693

Core Product Specifications and Parameters

Biological Background: TCTP/TPT1 Function and Localization

• TCTP (Translationally-controlled tumor protein), also known as Fortilin, histamine-releasing factor (HRF), and p23, is encoded by the TPT1 gene.
• TCTP is involved in calcium binding and microtubule stabilization. Related references: PMID:12167714 PMID:15162379 PMID:15958728
• TCTP acts as a negative regulator of TSC22D1-mediated apoptosis by interacting with and destabilizing TSC22D1 protein. Related references: PMID:18325344
• The protein localizes to the cytoplasm.
• TCTP is widely expressed in healthy and tumoral cells, including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, and various tumors; however, it is not detected in kidney and renal cell carcinoma (RCC). It is also expressed in placenta and prostate.
• Post-translational modifications include phosphorylation, consistent with its classification as a phosphoprotein.
• Alternative splicing produces multiple isoforms of TCTP.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of human TCTP/TPT1 (P13693). Consider using this region for epitope mapping or competition assays when designing experiments.
• This rabbit monoclonal antibody is suitable for WB, IP, IHC-P, and ELISA. For each application, optimize dilutions and include appropriate positive and negative controls.
• The antibody cross-reacts with human, mouse, and rat TCTP/TPT1. Validate specificity in the relevant species and tissue context before use.
• Since TCTP is a ~20kDa cytoplasmic protein, use appropriate lysis buffers and loading controls for Western blotting to ensure optimal detection.

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As a dedicated one-stop IVD raw-material supplier, CamelBio supports diagnostic manufacturers, laboratories, and research institutes with validated antibodies and ancillary reagents for cancer and apoptosis research. Our high-quality Anti-TCTP/TPT1 monoclonal antibody reflects our commitment to providing reliable tools for biomarker development and assay optimization. Explore our portfolio for comprehensive sourcing solutions from concept to clinic.