Anti-TCTA Polyclonal Antibody for WB, IF/ICC, ELISA - P57738

Core Product Specifications and Parameters

Biological Background: T-cell Leukemia Translocation-altered Gene Protein (TCTA) Function and Localization

• TCTA (T-cell leukemia translocation-altered gene protein) is a proto-oncogene originally identified through chromosomal rearrangement in T-cell leukemia. The protein is also known as T-cell leukemia translocation-associated gene protein.
• It is a small transmembrane protein with a calculated molecular weight of ~11 kDa, consisting of 103 amino acids.
• TCTA may play a role in cellular fusion during osteoclastogenesis, contributing to bone resorption and remodeling.
• The protein localizes to the membrane and possesses a single transmembrane helix, classifying it as an integral membrane protein.
• TCTA is expressed ubiquitously, with the highest levels in the kidney. At the protein level, it is detected in monocytes, osteoclasts, macrophages, synoviocytes, and synovial lining cells.
• Post-translational modifications include N-linked glycosylation and acetylation, which may regulate its function or stability.

Experimental Guidance and Technical Tips

• For Western blotting (WB), the predicted molecular weight is 11 kDa. Consider using high-concentration gels (e.g., 15%) and low-molecular-weight markers, along with careful transfer conditions to prevent loss of small proteins.
• In immunofluorescence/ICC, TCTA is a membrane protein. Employ appropriate permeabilization and blocking steps to ensure antibody access. Validate the staining pattern in known expressing cell types such as osteoclasts or macrophages.
• For ELISA, the antibody can be used as a detection reagent; however, optimal coating and detection conditions should be empirically determined for the specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, including validated polyclonal and monoclonal antibodies like this anti-TCTA antibody. Supporting bone biology and osteoclast-related research, we offer one-stop sourcing of antibody pairs, bulk ancillary reagents, and custom procurement for rare targets—streamlining your workflow from concept to clinic.