Anti-TCP1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P17987

Core Product Specifications and Parameters

Biological Background: TCP1 Function and Localization

• TCP1 (T-complex protein 1 subunit alpha, also known as CCT-alpha) is a core subunit of the chaperonin-containing T-complex (TRiC), a molecular chaperone that assists in the folding of various proteins, notably actin and tubulin, through ATP hydrolysis. Related references: PMID:25467444, PMID:36493755, PMID:35449234
• The TRiC complex, via TCP1, is critical for telomere maintenance by folding WRAP53/TCAB1, which regulates telomerase trafficking. Related references: PMID:25467444
• TCP1 participates in the assembly of the BBSome, a complex essential for ciliogenesis and vesicular transport to the cilium. Related references: PMID:20080638
• Within the cell, TCP1 is predominantly localized in the cytoplasm and cytosol, with additional presence at the centrosome, aligning with its roles in cytoskeletal dynamics and cell division.
• TCP1 is subject to post-translational modifications, including acetylation and phosphorylation, and features an ATP-binding domain vital for its chaperone activity.
• Clinically, mutations in the TCP1 gene are associated with neurological conditions such as epilepsy and intellectual disability, underscoring its importance in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 307–556 of human TCP1; sequence alignment with other CCT subunits is recommended to assess potential cross-reactivity.
• In Western blotting, expect a band near 60 kDa (UniProt calculated mass: 60.3 kDa); include appropriate controls for verification.
• For IHC-P, antigen retrieval conditions may need optimization; test on tissues with known TCP1 expression, such as testis or proliferative cells.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays.
• Reported cross-reactivity with mouse and rat should be confirmed in the end-user’s specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development by offering a comprehensive range of raw materials, from validated antibody pairs to bulk ancillary reagents. For research into protein folding, cytoskeletal dynamics, and chaperonin biology, this TCP1 antibody supports Western blotting, IHC-P, and ELISA applications. As your one-stop partner, we also assist in sourcing rare targets and custom antibody development, accelerating your projects from concept to clinic.