Anti-TCP1 beta/CCT2 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P78371

Core Product Specifications and Parameters

Biological Background: TCP1 beta/CCT2 Function and Localization

• TCP1 beta (CCT2, CCT-beta) is the beta subunit of the chaperonin-containing T-complex (TRiC), a group II chaperonin essential for protein folding in the cytoplasm.
• As part of TRiC, TCP1 beta participates in the ATP-dependent folding of newly synthesized actin and tubulin, critical for cytoskeletal integrity and cell division. Related references: PMID:25467444 PMID:36493755 PMID:35449234
• TRiC also folds WRAP53/TCAB1, a component required for telomere maintenance, highlighting its role in genome stability. Related references: PMID:25467444
• Additionally, TRiC contributes to the assembly of the BBSome, a multiprotein complex that regulates vesicular trafficking to the primary cilium, important for ciliary signaling. Related references: PMID:20080638
• TCP1 beta localizes to the cytoplasm, consistent with its function as a molecular chaperone.
• The protein features an ATP-binding domain and undergoes post-translational modifications such as acetylation and phosphorylation, which may regulate its chaperonin activity.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 206-535), which may enable recognition of both native and denatured TCP1 beta; however, cross-reactivity with other CCT subunits should be tested.
• For Western blot, a band corresponding to ~57 kDa is expected in human and mouse cell lysates. Validate with appropriate controls.
• For immunofluorescence, cytoplasmic staining should be observed; consider co-staining with organelle markers.
• In immunoprecipitation, this polyclonal antibody may efficiently capture the TCP1 beta subunit and associated TRiC complex components; optimization of lysis and wash conditions is recommended.
• For ELISA, the antibody can be used in both direct and sandwich formats; pairing with a monoclonal anti-TCP1 beta antibody may enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with integrated access to high-quality IVD raw materials, including validated antibody pairs, optimized recombinant proteins, and ancillary reagents. For TCP1 beta/CCT2 research, CamelBio offers a reliable sourcing solution for customized bulk antibody production, rare protein target reagents, and technical consultation to accelerate your assay development from concept to clinic.