Anti-TCP1 beta/CCT2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P78371

Core Product Specifications and Parameters

Biological Background: TCP1 beta/CCT2 Function and Localization

• TCP1 beta/CCT2, also known as CCT-beta or chaperonin containing T-complex polypeptide 1 subunit 2, is a subunit of the chaperonin-containing T-complex (TRiC), a heterooligomeric molecular chaperone complex essential for protein folding.
• As a component of TRiC, it facilitates ATP-dependent folding of nascent polypeptides, including cytoskeletal proteins actin and tubulin, ensuring proper cytoskeletal dynamics. Related references: PMID:25467444, PMID:36493755, PMID:35449234
• The TRiC complex, including CCT2, mediates the folding of WRAP53/TCAB1, a protein critical for telomere maintenance and Cajal body integrity. Related references: PMID:25467444
• TRiC may also participate in the assembly of the BBSome, a complex involved in ciliogenesis by regulating vesicular transport to the primary cilium. Related references: PMID:20080638
• TCP1 beta/CCT2 localizes predominantly in the cytoplasm, consistent with its role in co-translational and post-translational protein folding events.
• Post-translational modifications of CCT2 include acetylation, phosphorylation, and ubiquitination, which may regulate its activity, stability, or interactions within the TRiC complex.
• The protein belongs to the TCP-1 chaperonin family and is conserved across eukaryotes; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 436-535) of human TCP1 beta/CCT2; this region is conserved in mouse and rat, supporting cross-reactivity.
• For Western blot (WB), the predicted molecular weight is 57 kDa; use appropriate molecular weight markers and validate band specificity in the relevant sample system.
• For immunohistochemistry (IHC-P), consider antigen retrieval methods and validate with appropriate positive and negative controls.
• ELISA applications may require optimization of coating antigen and antibody dilution; consider pairing with a matched detection antibody.
• Since TCP1 beta/CCT2 is a cytoplasmic chaperone, prepare cytosolic or total protein extracts for optimal detection.
• The antibody is a rabbit monoclonal, ensuring batch-to-batch consistency and high specificity; consider its use in co-immunoprecipitation if validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with integrated access to high-quality IVD raw materials. In the field of molecular chaperones and proteostasis, we offer validated monoclonal antibodies like this anti-TCP1 beta/CCT2 rabbit mAb, alongside antibody pairs, recombinant proteins, and ancillary reagents. Our sourcing network supports the development of robust immunoassays for studying protein folding, cytoskeletal dynamics, and ciliogenesis pathways.