Anti-TCP1 alpha Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P17987

Core Product Specifications and Parameters

Biological Background: TCP1 Alpha Function and Localization

• TCP1 alpha, also known as T-complex protein 1 subunit alpha, CCT-alpha, or chaperonin containing T-complex polypeptide 1 subunit 1, is a subunit of the chaperonin-containing T-complex (TRiC).
• As a component of TRiC, TCP1 alpha facilitates the ATP-dependent folding of newly synthesized proteins, including actin and tubulin, ensuring proper cytoskeleton formation. Related references: PMID:25467444, PMID:36493755, PMID:35449234
• TRiC, with TCP1 alpha, mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. Related references: PMID:25467444
• This subunit may also play a role in BBSome assembly, a complex essential for ciliogenesis and vesicular transport to cilia. Related references: PMID:20080638
• TCP1 alpha localizes predominantly to the cytoplasm, cytosol, and centrosomes, consistent with its chaperone and cytoskeletal functions.
• Post-translational modifications include acetylation and phosphorylation, and mutations in TCP1 are associated with epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human TCP1 alpha, suggesting the antibody recognizes a linear epitope; consider this when designing antigen retrieval or denaturing conditions.
• For WB, validate the antibody in your specific human sample type as reactivity has been confirmed for human; a band around 60 kDa is expected.
• In IHC-P, antigen retrieval optimization may be necessary; test antibody dilutions on control tissues with known TCP1 expression.
• For ELISA, pair with appropriate secondary detection reagents and consider using recombinant TCP1 alpha as a positive control.
• Since this is a rabbit monoclonal, it offers batch-to-batch consistency; still, validate performance in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, connecting diagnostic manufacturers and research groups with validated reagents like this anti-TCP1 alpha antibody. We offer a full portfolio—from optimized monoclonal/polyclonal antibodies and validated antibody pairs to bulk ancillary reagents—tailored for workflows investigating chaperone biology, protein folding, and cytoskeletal dynamics. Through CamelBio’s one-stop platform, you gain efficient access to high-quality materials that help accelerate your assay development from concept to clinic.