Anti-TCOF1 Polyclonal Antibody for WB and ELISA - Q13428

Core Product Specifications and Parameters

Biological Background: TCOF1 Function and Localization

• Treacle protein, encoded by the TCOF1 gene, is also known as Treacher Collins syndrome protein.
• Functions as a nucleolar regulator of RNA polymerase I and is essential for neural crest specification, connecting RNA polymerase I with ribosomal processing and modification enzymes to remodel translational programs. Related references: PMID:12777385 PMID:26399832
• Localizes to the nucleolus within the nucleus.
• Undergoes multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination (isopeptide bond formation).
• Classified as a disease variant protein; mutations are linked to Treacher Collins syndrome.
• Exhibits alternative splicing, producing distinct isoforms.
• Predicted molecular weight of approximately 152 kDa, consistent with the observed protein weight.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant fragment covering amino acids 659–958. Consider the immunogen location when designing experiments.
• For Western blotting, note the large molecular weight of TCOF1 (~152 kDa). Use a low-percentage polyacrylamide gel and extend transfer times to ensure efficient detection.
• For ELISA, optimize the coating antigen and antibody dilution in the relevant sample matrix. Validate specificity using appropriate controls.
• Although this antibody is reported to react with human TCOF1, cross-reactivity with other species has not been tested; validate in your experimental system if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material supplier supporting diagnostic developers, research laboratories, and biotech firms with a comprehensive portfolio of high-quality antibodies and reagents. For studies involving TCOF1 and nucleolar function, we provide validated polyclonal antibodies like this one, and can assist in sourcing matched antibody pairs, recombinant proteins, and ancillary reagents. From concept to clinic, CamelBio offers one-stop access to the materials and technical consulting needed to advance your diagnostic and research programs.