Anti-TCN2 (Transcobalamin-2) Rabbit Polyclonal Antibody for WB, ELISA - P20062

Core Product Specifications and Parameters

Biological Background: Transcobalamin-2 Function and Localization

• Transcobalamin-2 (TCN2), also known as TC II, is a secreted vitamin B12 (cobalamin)-binding protein that functions as the primary transporter of cobalamin to cells.
• It mediates the delivery of cobalamin via receptor-mediated endocytosis after binding to the transcobalamin receptor.
• The protein contains a signal peptide and is secreted into the bloodstream, where it binds cobalamin with high affinity.
• TCN2 is characterized by disulfide bonds and a cobalt-binding site, essential for its transport function.
• Alternative splicing of the TCN2 gene produces multiple transcript variants.
• Genetic variants in TCN2 are associated with diseases related to cobalamin deficiency.
• The protein has been directly sequenced and its three-dimensional structure determined.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 19–250, encompassing the signal peptide and cobalamin-binding domain; this region may detect both full-length and secreted TCN2.
• For Western blotting, the predicted molecular weight is 48 kDa; use reducing conditions to ensure detection of the monomeric form.
• When performing ELISA, validate the antibody’s sensitivity using recombinant TCN2 protein or serum samples with known cobalamin levels.
• Cross-reactivity with mouse TCN2 suggests potential utility in rodent models; however, confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive supply chain for IVD manufacturers and researchers, offering validated antibody pairs, bulk ancillary reagents, and customized raw-material sourcing. For studies on cobalamin metabolism and vitamin B12 transport, this TCN2 polyclonal antibody complements a range of related reagents to support your diagnostic development. Contact our team to streamline your procurement and accelerate your project timeline.