Anti-TCIRG1 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - Q13488

Core Product Specifications and Parameters

Biological Background: TCIRG1/V-ATPase Subunit a3 Function and Localization

• TCIRG1 (T-cell immune regulator 1, also known as ATP6V0A3, OC116, a3) encodes the 116 kDa subunit a3 of the V0 complex of vacuolar H+-ATPase (V-ATPase), a multisubunit enzyme responsible for acidifying intracellular compartments and, in some cell types, the extracellular environment.
• The V-ATPase consists of a peripheral V1 complex that hydrolyzes ATP and a membrane-integral V0 complex that translocates protons; the a3 subunit is essential for proton translocation across membranes.
• TCIRG1 is directly involved in T-cell activation, as supported by evidence showing its role in immune cell regulation (PubMed:10329006).
  Related references: PMID:10329006
• Mutations in TCIRG1 are associated with autosomal recessive osteopetrosis, highlighting its critical function in osteoclast-mediated bone resorption and pH regulation.
• The protein exhibits isoform diversity: isoform long is highly expressed in osteoclastomas, while isoform short is predominantly expressed in the thymus.
• Subcellularly, TCIRG1 is a transmembrane protein localized to the membrane of intracellular vesicles and the plasma membrane, where it helps maintain pH homeostasis.
• Key biological keywords include alternative splicing, disease variant, hydrogen ion transport, ion transport, membrane, osteopetrosis, and transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-356 of human TCIRG1, a large N-terminal cytoplasmic domain. This region is likely well-exposed and may facilitate recognition in both native and denatured conditions, supporting applications such as WB and IHC-P.
• For Western blotting, a band around 93 kDa is expected; consider using appropriate denaturing conditions as the protein may aggregate due to transmembrane domains.
• In immunofluorescence (IF/ICC and IF-P), permeabilization is required to detect the intracellular membrane-bound protein. Use appropriate controls to verify specificity.
• When using IHC-P, antigen retrieval methods (e.g., heat-induced epitope retrieval) may improve staining, especially in formalin-fixed tissues.
• Cross-reactivity with mouse and rat orthologs enables potential use in rodent models, but validate expression and specificity in the relevant tissue or cell line.
• For ELISA, the antibody may be suitable for capturing or detecting the recombinant immunogen; however, note that the native protein's multimeric state could affect binding in complex samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop platform for IVD raw materials, connecting you to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. TCIRG1, a key player in osteoclast biology, immune regulation, and proton transport, is among the specialized targets we support. Our supply capabilities help streamline your workflow from concept to clinical application.