Anti-TCIRG1 Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q13488

Core Product Specifications and Parameters

Biological Background: TCIRG1 Function and Localization

• TCIRG1 encodes the 116 kDa subunit a3 of the V0 complex of vacuolar H+-ATPase (V-ATPase), also known as OC-116 kDa, TIRC7, and ATP6V0A3.
• V-ATPase is a multi-subunit enzyme that acidifies intracellular compartments and, in some cells, the extracellular environment. The V0 complex mediates proton translocation across membranes.
• TCIRG1 is directly involved in T-cell activation, as shown by functional studies (PubMed:10329006).
  Related references: PMID:10329006
• The protein is a transmembrane protein localized to the membrane.
• Isoform long is highly expressed in osteoclastomas, while isoform short is highly expressed in the thymus, suggesting tissue-specific roles.
• Keywords: Alternative splicing, Disease variant, Hydrogen ion transport, Ion transport, Osteopetrosis, Transmembrane. Mutations in TCIRG1 are associated with osteopetrosis.
• The predicted molecular weight is approximately 93 kDa (UniProt: 92968 Da), consistent with the observed 93 kDa band.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-130) of human TCIRG1. This region may provide specificity for the long isoform; consider validating isoform detection in relevant samples.
• For Western blotting, a band around 93 kDa is expected. Use appropriate molecular weight markers and validate in known positive samples such as osteoclastoma or thymus lysates.
• For immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), membrane localization should be observed. Include no-primary antibody controls to assess background.
• The antibody is suitable for ELISA; consider its use in screening applications where native or recombinant TCIRG1 is coated.
• Cross-reactivity with mouse and rat allows for multi-species studies; verify performance in these species under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This TCIRG1 antibody targets a key V-ATPase subunit involved in osteoclast function and T-cell activation, making it valuable for bone metabolism and immunology research. We offer validated antibody pairs, bulk monoclonal/polyclonal reagents, and custom sourcing for rare targets to streamline your assay development.