Anti-TCF7L1 Polyclonal Antibody for WB and ELISA - Q9HCS4

Core Product Specifications and Parameters

Biological Background: TCF7L1 Function and Localization

• TCF7L1, also known as TCF-3 or HMG box transcription factor 3, is a member of the TCF/LEF family of high-mobility group (HMG) box transcription factors that mediate canonical Wnt signaling.
• In the absence of Wnt/β-catenin signaling, TCF7L1 acts as a transcriptional repressor by binding to Wnt-responsive DNA elements; upon pathway activation and β-catenin (CTNNB1) accumulation, it switches to an activator, promoting target gene expression.
• TCF7L1 is essential for terminal differentiation of epidermal cells, formation of keratohyalin granules, and establishment of the skin barrier function, as demonstrated by similarity studies.
• The protein localizes to the nucleus, consistent with its role as a DNA-binding transcription factor.
• TCF7L1 is predominantly expressed in hair follicles, skin keratinocytes, and at lower levels in stomach epithelium, highlighting its tissue-specific regulatory functions.
• It down-regulates NQO1 expression, which has been linked to increased resistance to mitomycin C, suggesting a role in chemoresistance.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 260–340 of human TCF7L1, a region that may provide specific recognition of the target; consider blocking with the immunogen fragment to confirm specificity.
• For Western blot, the predicted molecular weight is approximately 63 kDa; use lysates from Wnt-activated cells or epidermal keratinocytes as positive controls.
• In ELISA, this antibody can be used for capture or detection; validate in the relevant sample system, keeping in mind cross-reactivity with mouse and rat TCF7L1.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering reliable access to validated antibodies and ancillary reagents. This TCF7L1 polyclonal antibody supports research into Wnt signaling and epidermal biology. Beyond individual antibodies, CamelBio provides bulk custom antibody production, monoclonal antibody development, and sourcing of rare-target reagents to accelerate your diagnostic assay development from concept to clinic.