Anti-TCF4/TCF7L2 Polyclonal Antibody for WB, ELISA - Q9NQB0

Core Product Specifications and Parameters

Biological Background: TCF4/TCF7L2 Function and Localization

• Transcription factor 7-like 2 (TCF7L2), also known as TCF-4, TCF4, or hTCF-4, is a member of the HMG box transcription factor family.
• Functions as a key effector of the Wnt signaling pathway: acts as a transcriptional repressor in the absence of β-catenin (CTNNB1) and as an activator upon β-catenin binding.
• Binds the Tcf motif 5'-CCTTTGATC-3' in target gene promoters; regulates expression of MYC and other Wnt-responsive genes.
• Necessary for maintenance of the epithelial stem-cell compartment in the small intestine; expression of dominant-negative mutants induces G1 cell-cycle arrest.
• Primarily localizes to the nucleus and PML nuclear bodies.
• Detected in small intestinal epithelium (highest at crypt top), colon epithelium, colon cancer, and mammary gland epithelium and carcinomas.
• Post-translationally modified by phosphorylation and ubiquitination (UBL conjugation); contains isopeptide bonds.
• Annotated with the keyword 'Diabetes mellitus', indicating disease relevance.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fragment corresponding to amino acids 124–188 of human TCF4/TCF7L2 (isoform NP_001354872.1). Due to alternative splicing, this epitope may be present in some but not all isoforms; verify expression in your model system.
• For Western blotting, use nuclear-enriched lysates from human or mouse intestinal or colon epithelial cells/tissues to enrich for TCF4/TCF7L2. Expect a band around 68 kDa; multiple bands may appear due to isoform diversity and post-translational modifications.
• For ELISA, consider using recombinant TCF4/TCF7L2 fragment as coating antigen and validate with known positive and negative control sera or purified antibodies.
• Optimize antibody dilution and incubation conditions for each application; include appropriate controls (e.g., siRNA knockdown or knockout cell lysates) to confirm specificity.
• Cross-reactivity with human and mouse has been indicated; reactivity with other species should be tested if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material sourcing partner, empowering diagnostic manufacturers, laboratories, and research institutes from concept to clinic. For TCF4/TCF7L2 and other Wnt pathway targets, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and expedited sourcing of rare targets to support your assay development and research programs.